Loading...

Ultra-LEAF™ Purified anti-human TWEAK (CD255) Antibody

Pricing & Availability
Clone
A18102K (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor superfamily, member 12; TNFSF12, YNF-related weak inducar of poptosis, Apo-3 ligand (Apo3L)
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A18102K_ULEAF_TWEAK_Neut_082825
Recombinant Human TNFRSF12A-Fc Chimera (Cat. No. 769104) (black circles) binds to immobilized Recombinant Human TWEAK (CD255) (Cat. No. 566402, 566404). Ultra-LEAF™ Purified anti-human TWEAK (clone A18102K, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified mouse IgG2b, κ Isotype Control Antibody (Cat. No. 401215) (gray triangles) has no inhibitory effect. This antibody blocks the binding of 70 ng/mL Recombinant Human TNFRSF12A-Fc Chimera to 0.5 µg/mL of immobilized Recombinant Human TWEAK (CD255). The ND50 range to observe this effect is: 0.8 – 8.0 µg/mL.
  • A18102K_ULEAF_TWEAK_Neut_082825
    Recombinant Human TNFRSF12A-Fc Chimera (Cat. No. 769104) (black circles) binds to immobilized Recombinant Human TWEAK (CD255) (Cat. No. 566402, 566404). Ultra-LEAF™ Purified anti-human TWEAK (clone A18102K, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified mouse IgG2b, κ Isotype Control Antibody (Cat. No. 401215) (gray triangles) has no inhibitory effect. This antibody blocks the binding of 70 ng/mL Recombinant Human TNFRSF12A-Fc Chimera to 0.5 µg/mL of immobilized Recombinant Human TWEAK (CD255). The ND50 range to observe this effect is: 0.8 – 8.0 µg/mL.
Cat # Size Price Quantity Check Availability Save
648844 100 µg SEK2856
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648845 1 mg SEK6678
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TWEAK (TNFSF12) is a "TNF-like weak inducer" of apoptosis through a non-death domain-dependent mechanism. TWEAK is a type II membrane protein which exhibits a single internal hydrophobic domain of 27 amino acids in the N-terminal region. TWEAK is proteolytically cleaved to produce a soluble cytokine that signals as a trimerized molecule. Fibroblast growth factor-inducible 14 (Fn14)/TWEAKR has been described as a receptor for TWEAK, and it is associated with proliferation of endothelial cells and angiogenesis. However, TWEAK mediates signal transduction and linear differentiation of monocyte/macrophage cells lacking Fn14/TWEAKR, suggesting that such cells contain an alternative TWEAK receptor. Elevated levels of TWEAK and/or Fn14 have been found to be associated with the pathogenesis of rheumatoid arthritis, skeletal muscle wasting, systemic lupus erythematosus, multiple sclerosis, stroke, neuroinflammation and neurodegeneration, and several types of cancer. The pathological functions of TWEAK are primarily attributed to its ability to induce the expression of several proinflammatory cytokines, chemokines, cell adhesion molecules, and matrix-degrading enzymes mainly through the activation of NF-κB, a major proinflammatory transcription factor. It has been described that CD163 (a scavenger receptor) might be acting as a receptor decoy for the ligand TWEAK.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TWEAK
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing human TWEAK (Cat. No. 566402, 566404) and inhibiting its binding to human TNFRSF12A-Fc Chimera (Cat. No. 769104) at 70 ng/mL. ND50 range: 0.8 – 8.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Homotrimer
Distribution

Monocytes, macrophages, and TWEAK transcripts are widely expressed and thus abundant in most tissues.

Function
TWEAK induces interleukin-8 synthesis in several cell lines, and IL-6 and IL-8 in human astrocytes in vitro. TWEAK acts cooperatively with bFGF to promote EC proliferation, migration and capillary tube morphogenesis.
Interaction
Endothelial cells, macrophages, astrocytes.
Ligand/Receptor
Fibroblast growth factor inducible 14 (Fn14)/TWEAKR (CD266)
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References
  1. Chicheportiche Y, et al. 1997. J. Biol. Chem. 272:32401.
  2. Wiley SR, et al. 2001. Immunity. 15:837.
  3. Jakubowski A, et al. 2002. J. Cell. Sci. 115:267.
  4. Bover LC, et al. 2007. J. Immunol. 178:8183.
  5. Kumar M, et al. 2009. J. Immunol. 182:2439.
  6. Van Gorp H, et al. 2010. Mol. Immunol. 47:1650.
Gene ID
NA
UniProt
View information about TWEAK on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08-28-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account