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Purified anti-human CD16 Antibody

Pricing & Availability
Clone
W18182B (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W18182B_PURE_CD16_FC_052925
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) FITC and purified anti-human CD16 (clone W18182B) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG APC.
  • W18182B_PURE_CD16_FC_052925
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) FITC and purified anti-human CD16 (clone W18182B) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG APC.
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310009 100 µg SEK882
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD16 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Activ - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18182B can completely block the binding of clone B73.1 on target cells.

Clone W18182B does not affect the binding of clones 3G8 or W18182A on target cells.

Clone W18182B is able to activate NK cells and induce degranulation.

RRID
AB_3720492 (BioLegend Cat. No. 310009)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

Expressed on natural killer cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor
Ligand/Receptor
IgG Fc (Fcγ)
Cell Type
Granulocytes, Neutrophils, NK cells
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
  2. Ravetch JV and Bolland S. 2001. Annu Rev Immunol. 19:275.
Gene ID
2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05-29-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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