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PE anti-human CD303 (BDCA-2) Antibody

Pricing & Availability
Clone
W19421D (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19421D_PE_CD303_Antibody_021224
Human peripheral blood mononuclear cells were stained with anti-human CD123 (clone 6H6) APC and anti-human CD303 (BDCA-2) (clone W19421D) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown was gated on the lymphocyte and monocyte populations.
  • W19421D_PE_CD303_Antibody_021224
    Human peripheral blood mononuclear cells were stained with anti-human CD123 (clone 6H6) APC and anti-human CD303 (BDCA-2) (clone W19421D) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown was gated on the lymphocyte and monocyte populations.
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349753 25 tests SEK1227
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349754 100 tests SEK2680
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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CLEC4C-transfectants (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W19421D shows minimal or no non-specific binding to the monocyte compared to both the current catalogue clones (201A & S19008G).

Clone W19421D does not cross block with clone 201A.

Clone W19421D cross blocks with clone S19008G.

RRID
AB_3720483 (BioLegend Cat. No. 349753)
AB_3720483 (BioLegend Cat. No. 349754)

Antigen Details

Structure
Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution

Plasmacytoid dendritic cells

Function
Antigen capture, inhibition of IFN α/β production
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
  2. Graham LM and Brown GD. 2009. Cytokine. 48:148.
  3. Riboldi E, et al. 2011. J Biol Chem. 2011; 286:35329-33.
  4. Shinde R, et al. 2018. Nat Immunol. 2018; 19:571-582. 
Gene ID
170482 View all products for this Gene ID
UniProt
View information about CD303 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02-12-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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