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Alexa Fluor® 647 anti-NF-κB p65 Phospho (Ser529) Antibody

Pricing & Availability
Clone
A21012B (See other available formats)
Regulatory Status
RUO
Other Names
NF-kB p65, RelA,P65, NFKB3, Nuclear Factor NF-Kappa-B P65 subunit, Transcription Factor P65, NF-Kappa-B Transcription Factor P65, RELA Proto-Oncogene NF-KB subunit
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A21012B_A647_NF-kB_Antibody_1_012126
Human PBMCs were left untreated (negative control, open histogram) or treated (positive control, purple filled histogram) with Cell Activation Cocktail without Brefeldin A (Cat. No. 423301). Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with Alexa Fluor® 647 anti-NF-κB p65 Phospho (Ser529) (clone A21012B) or Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400102).
  • A21012B_A647_NF-kB_Antibody_1_012126
    Human PBMCs were left untreated (negative control, open histogram) or treated (positive control, purple filled histogram) with Cell Activation Cocktail without Brefeldin A (Cat. No. 423301). Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with Alexa Fluor® 647 anti-NF-κB p65 Phospho (Ser529) (clone A21012B) or Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400102).
Compare all formats See Alexa Fluor® 647 spectral data
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614155 25 tests SEK1428
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614156 100 tests SEK3444
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Description

NF-κB p65, also known as NFKB3 and RelA, is one of five members of the nuclear factor κB (NF-κB)/Rel family. Inactive NF-κB p65 is sequestered in the cytoplasm as a complex with IκB inhibitory proteins. Upon activation of the NF-κB pathway, IκB proteins are degraded, thus releasing NF-κB p65 to the nucleus. Binding of NF-κB p65 to transcriptional cofactors is induced via phosphorylation on serines 276, 529, 536, and 471. As a transcription factor, NF-κB p65 is vital to cell proliferation, immune response, survival, and apoptosis. Through regulation of chondrocyte and osteoblast differentiation and survival, NFκB p65 has a crucial role in skeletal development. Overexpressed in several cancers, NF-κB p65 functions as an oncogene and has been implicated in tumorigenesis, metastasis, tumor angiogenesis, and chemoresistance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human NF-κB p65 phosphorylated at serine 529
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)

Antigen Details

Structure
NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
Distribution

Ubiquitously expressed/ Cytoplasm and Nucleus

Function
Transcription, RAS pathway, host-virus interaction, oncogene
Interaction
NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Innate Immune Signaling, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Giridharan S and Srinivasan M. 2018. J Inflamm Res. 11:407-419.
  2. Ren C, et al. 2022. Cell Death Differ. 29:381-392.
  3. Yu S, et al. 2020. FEBS Lett. 594:13681378.
Gene ID
5970 View all products for this Gene ID
UniProt
View information about NF-kappaB p65 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01-21-2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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