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Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. MOPC-21_A555_MouseIgG1_ICC_092225
HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were stained with Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl (clone MOPC-21) only (panel A) or Purified anti-Histone H3 (C-terminus) Recombinant (Cat. No. 646852), followed by incubation with Alexa Fluor® 555 Goat anti-mouse IgG (Cat. No. 405324). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 25 µm
  • A. MOPC-21_A555_MouseIgG1_ICC_092225
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were stained with Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl (clone MOPC-21) only (panel A) or Purified anti-Histone H3 (C-terminus) Recombinant (Cat. No. 646852), followed by incubation with Alexa Fluor® 555 Goat anti-mouse IgG (Cat. No. 405324). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 25 µm
  • B. MOPC-21_A555_MouseIgG1_IHC-P_092225
    IHC staining of Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl (clone MOCP-21) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) or with (panel B) Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl (clone MOCP-21), or Purified anti-Histone H3 (C-terminus) (Cat. No. 646852) followed by incubation with Alexa Fluor® 555 Goat anti-mouse IgG (Cat. No. 405324) (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective and merged (panels D, E, and F). Scale bar: 50 µm
Compare all formats See Alexa Fluor® 555 spectral data
Cat # Size Price Quantity Check Availability Save
402453 100 µg SEK2016
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Certificate of Analysis online tool to find the exact concentrations of your lots of product.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

Application References
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
RRID
AB_3720602 (BioLegend Cat. No. 402453)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA,ChIP
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Biotin Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl MOPC-21 FC,ICFC
TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
FITC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
PE Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
FITC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl MOPC-21 SB
Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Alexa Fluor® 660 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark PLUS UV395™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Spark Red™ 718 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-B1471 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICPG
Spark PLUS B550™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
TotalSeq™-C1471 Mouse IgG1, κ Isotype Ctrl Antibody MOPC-21 ICPG
Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICC,IHC-P
Spark PLUS B488™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Go To Top Version: 1    Revision Date: 09-22-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified Mouse IgG1, κ Isotype Ctrl

    MOPC-21_ChIP_IgG1-k_control_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • APC Mouse IgG1, κ Isotype Ctrl

  • Biotin Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl

  • APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • APC Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl

  • Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl

  • Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl

  • Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • FITC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • PE Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (FC)

  • PE Mouse IgG1, κ Isotype Ctrl (FC)

  • FITC Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl

  • PerCP Mouse IgG1, κ Isotype Ctrl

    MOPC-21_PerCP_122607
    Human peripheral blood lymphocytes stained with MOPC-21 PerC...

  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl

  • Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl

  • GMP PE Mouse IgG1, κ Isotype Ctrl

  • GMP FITC Mouse IgG1, κ Isotype Ctrl

  • GMP APC Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl

  • Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 660 Mouse IgG1, κ Isotype Ctrl

  • Spark PLUS UV395™ Mouse IgG1, κ Isotype Ctrl

  • Spark Red™ 718 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-B1471 Mouse IgG1, κ Isotype Ctrl

  • Spark PLUS B550™ Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-C1471 Mouse IgG1, κ Isotype Ctrl Antibody

  • Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl

    A. MOPC-21_A555_MouseIgG1_ICC_092225
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801)...
    B. MOPC-21_A555_MouseIgG1_IHC-P_092225
    IHC staining of Alexa Fluor® 555 Mouse IgG1, κ Isotype Ctrl ...

  • Spark PLUS B488™ Mouse IgG1, κ Isotype Ctrl

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