Alexa Fluor® 488 anti-Podoplanin (Lymphatic Endothelial Marker), Podoplanin, D2-40

Pricing & Availability

Clone: D2-40 (See other available formats)
Regulatory Status: RUO
Other Names: Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype: Mouse IgG1, κ
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Product Citations: publications

D2-40_A488_Podoplanin_Antibody_1_111325 — LN-319 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 488 anti-Podoplanin (Lymphatic Endothelial Marker) (clone D2-40) or Alexa Fluor® 488 Mouse IgG1, κ isotype control (dashed histogram) (Cat No. 400134).

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916611

25 tests

SEK1176

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916612

100 tests

SEK2940

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Description

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: M2A protein derived from human germ cell tumors.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
RRID: AB_3720619 (BioLegend Cat. No. 916611)
AB_3720619 (BioLegend Cat. No. 916612)

Antigen Details

Structure: 35 kD
Distribution: Membrane and plasma membrane
Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction: Cytoskeletal signaling
Biology Area: Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID: 10630 View all products for this Gene ID
UniProt: View information about Podoplanin on UniProt.org

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Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All Podoplanin Reagents Request Custom Conjugation

Description	Clone	Applications
D2-40 Lymphatic Endothelial Marker Monoclonal Antibody	D2-40	IHC
Purified anti-Podoplanin (Lymphatic Endothelial Marker)	D2-40	IHC-P,WB,ICFC,ICC,ELISA Capture
Alexa Fluor® 594 anti-Podoplanin/Lymphatic Endothelial Marker	D2-40	IHC-P
Alexa Fluor® 647 anti-Podoplanin (Lymphatic Endothelial Marker)	D2-40	IHC-P
Alexa Fluor® 488 anti-Podoplanin (Lymphatic Endothelial Marker)	D2-40	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11-13-2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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