StarBright UltraViolet 740 anti-human TIGIT (VSTM3) antibody, TIGIT (VSTM3), A15153G

Pricing & Availability

Clone: A15153G (See other available formats)
Regulatory Status: RUO
Other Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype: Mouse IgG2a, κ
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Product Citations: publications

A15153G_SBUV740_TIGIT_FC_060525 — Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) FITC and anti-human TIGIT (VSTM3) (clone A15153G) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right). Data shown are gated on the lymphocyte population.

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373749

25 tests

135€

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373750

100 tests

300€

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human TIGIT.
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking¹.

Antigen Details

Structure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
Function: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type: NK cells, T cells, Tfh, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.
Gene ID: 201633 View all products for this Gene ID
UniProt: View information about TIGIT on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All TIGIT (VSTM3) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TIGIT (VSTM3)	A15153G	FC,Block
APC/Fire™ 750 anti-human TIGIT (VSTM3)	A15153G	FC
APC anti-human TIGIT (VSTM3)	A15153G	FC
PE anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 421™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 605™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Biotin anti-human TIGIT (VSTM3)	A15153G	FC
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)	A15153G	FC
Alexa Fluor® 647 anti-human TIGIT (VSTM3)	A15153G	FC
TotalSeq™-A0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-B0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-C0089 anti-human TIGIT (VSTM3)	A15153G	PG
KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)	A15153G	FC
APC/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 510™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody	A15153G	FC
TotalSeq™-D0089 anti-human TIGIT (VSTM3)	A15153G	PG
Brilliant Violet 711™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 640 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 810 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine5 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)	A15153G	FC
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)	A15153G	FC
PE/Fire™ 700 anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 650™ anti-human TIGIT (VSTM3) Antibody	A15153G	FC
PE/Fire™ 744 anti-human TIGIT (VSTM3) Antibody	A15153G	FC
Brilliant Violet 750™ anti-human TIGIT (VSTM3)	A15153G	FC
StarBright UltraViolet 740 anti-human TIGIT (VSTM3)	A15153G	FC
Spark PLUS V475™ anti-human TIGIT (VSTM3)	A15153G	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06-05-2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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