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Purified anti-ZO-2 Antibody

Pricing & Availability
Clone
W23248A (See other available formats)
Regulatory Status
RUO
Other Names
TJP2, Zona Occludens protein 2, TJP-2, Tight Junction Protein 2, Tight junctionprotein ZO-2, Zonula occludens protein 2, X104
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1 - W23248A_Pure_ZO-2_Antibody_2_110425
IHC staining with Purified anti-ZO-2 antibody on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-ZO-2 antibody (clone W23248A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • 1 - W23248A_Pure_ZO-2_Antibody_2_110425
    IHC staining with Purified anti-ZO-2 antibody on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-ZO-2 antibody (clone W23248A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • 2 - W23248A_Pure_ZO-2_Antibody_3_110425
    A-431 cells (top panels, positive control) or A-549 cells (bottom panels, negative control) were fixed and permeabilized with PFA and triton X-100, and then intracellularly stained with Purified anti-ZO-2 (clone W23248A) (right panels) or Purified Rat IgG2a,  Isotype Control (Cat No. 400502)(left panels) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Cells were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • 3 - W23248A_Pure_ZO-2_Antibody_1_110425
    Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-ZO-2 antibody (clone W23248A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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648892 25 µg 152€
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648893 100 µg 400€
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Description

The ZO-2 protein is a key component of tight junctions. It plays a role in maintaining cell polarity and barrier function. ZO-2 interacts with other tight junction proteins like claudins and occludins and participates in signaling pathways that influence gene expression and cell proliferation. Its expression is regulated by cellular stress, cytokines, and developmental cues. Mutations in TJP2 have been associated with several diseases, including progressive familial intrahepatic cholestasis type 4 (PFIC4), familial hypercholanemia, and autosomal dominant non-syndromic hearing loss (DFNA51).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

For use of this antibody in Immunocytochemistry (ICC), we recommendf ixation and permeabilization using PFA-Triton X-100 or PFA-ice-cold methanol. We do not recommend ice-cold methanol only.  

Antigen Details

Structure
The human ZO-2 protein consists of 1190 amino acids and has a molecular weight of approximately 133.9 kDa.
Distribution

Tight Junction/cellular membrane/nucleus

Interaction
ZO-1, ZO-3, Occludin, Claudins, Band 4.1, USP53, SAFB, ZASP
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Structure
Molecular Family
Adaptor Proteins, Adhesion Molecules, Gap Junction Proteins, Scaffold Proteins
Antigen References
  1. Gonzales-Mariscal L, et al. 2019. Int J Mol Sci. 20(17):4128
  2. Xu J, et al. 2021. Gastroenterology. 160(6):2103.
  3. Kim HS, et al. 2023. Int J Mol Sci. 24(10):8801
  4. Ma X, et al. 2025. Redox Rep. 30(1):2493556
Gene ID
9414 View all products for this Gene ID
UniProt
View information about ZO-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-04-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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