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Direct-Blot™ HRP anti-E. coli RNA Sigma 70 Recombinant Antibody

Pricing & Availability
Clone
2G10.RecM1 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase sigma factor RpoD, sigma 70, sigma D, σ70, P00579
Isotype
Mouse IgG1, κ
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Product Citations
publications
2G10RecM1_DirectBlotHRP_EColiRNA_WB_123025
Whole cell extracts (15 µg total protein per lane) from indicated cells and cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Direct-Blot™ HRP anti-E. Coli RNA Sigma 70 Recombinant (clone 2G10.RecM1) overnight at 4°C. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426315) was used as a detection agent. Lane M: Prime-Step™ Prestained Broad Range Protein Ladder (Cat. No. 773302)
  • 2G10RecM1_DirectBlotHRP_EColiRNA_WB_123025
    Whole cell extracts (15 µg total protein per lane) from indicated cells and cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Direct-Blot™ HRP anti-E. Coli RNA Sigma 70 Recombinant (clone 2G10.RecM1) overnight at 4°C. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426315) was used as a detection agent. Lane M: Prime-Step™ Prestained Broad Range Protein Ladder (Cat. No. 773302)
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666054 100 µL 288€
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Description

Most prokaryotes such as E. coli express a single core RNA polymerase to transcribe RNA from DNA. RNA Sigma 70, also known as rpoD, is the major housekeeping member of the sigma factor subunit family which are required for core RNA polymerase to bind to promoters and initiate transcription. RNA sigma 70 is also required for the unwinding of DNA strands to allow access to RNA polymerase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:1000 – 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody is derived from the original clone 2G10. We sequenced the complementarity-determining regions (CDRs) from the 2G10 hybridoma and grafted them into a mouse IgG1, κ Fc framework. Clone 2G10 specifically recognizes the major sigma factor across multiple bacterial species but shows no reactivity against Borrelia burgdorferi. Clone 2G10 is known to target epitope 470-486 in the major sigma factor of E. coli.

Antigen Details

Structure
RNA Sigma 70 is a 70 kD transcription factor of E. coli RNA polymerase.
Distribution

Bacterial cytoplasm

Interaction
Core RNA polymerase subunits (RpoA, RpoB, RpoC, RpoZ)
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Gruber T, et al. 2003. Annual review of microbiology. 441-66. PMID: 14527287
Gene ID
947567 View all products for this Gene ID
UniProt
View information about Sigma 70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12-30-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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