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- Other Names
- OSM
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Cell-Vive™ GMP Recombinant Human Oncostatin M induces the proliferation of human erythroleukemic TF-1 cells in a dose dependent manner.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 555214 | 25 µg | 592€ | ||||
| 555216 | 100 µg | 1480€ | ||||
Human Oncostatin M (OSM) was initially isolated from supernatant of U937 cells treated with PMA. It was identified by its property to inhibit the proliferation of A375 melanoma cells and other human tumor cells. Bioinformatic homology analysis reveals that OSM has a similar composition and structure to LIF, G-CSF, and IL-6. OSM and LIF are located on human chromosome 22, and additional evidence suggests that these genes were derived by duplication of a common ancestral gene. In fact, many of its biological functions are shared with LIF. OSM binds two types of OSM receptor (OSMR) complexes. The type I receptor complex is composed of gp130 (IL-6 signal transducer) and LIF receptor β (LIFRβ) subunits. The type II receptor is constituted by the gp130 receptor and the OSMR β-chain. OSM also shares the OSMRβ-chain with the IL-31 receptor complex. The OSMRβ subunit is expressed by fibroblast, endothelial, hepatic, lung, and hematopoietic cells. The proinflammatory properties of OSM have been reported in skin, adipose tissue, lung, heart, and liver tissues. OSM triggers acute phase protein synthesis in hepatocytes. OSM is a potent inhibitor of keratinocyte proliferation and decreases the expression of both early and late keratinocyte differentiation markers. OSM transcripts are elevated in skin from psoriasis and atopic dermatitis patients, as compared with healthy skin. OSM and its receptor complexes play a key role in cutaneous inflammatory responses. In addition, OSM expression is increased in colonic biopsies of patients with active inflammatory bowel disease.
Product Details
BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/μg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human Oncostatin M, amino acids (Ala26-Arg221) (Accession# NM_020530), was expressed in E. coli.
- Molecular Mass
- The 196 amino acid recombinant protein has a predicted molecular mass of approximately 22.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 20 kD and 22 kD, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Ala.
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N-terminal
Sequence Analysis - Ala-Ala-Ile-Gly-Ser-(Cys)-Ser-Lys-Glu-Tyr
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.1 μm filtered protein solution is in PBS, 5 mM Citric Acid, pH 3.5.
- Endotoxin Level
- Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
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Residual Host Cell
DNA Content - ≤ 0.002 ng/µg
- Residual Host Cell Protein Content
- ≤ 0.500 ng/μg by ELISA
- Concentration
- 25 μg and 100 μg sizes are bottled at 0.5 mg/mL
- Storage & Handling
- Unopened vials can be stored at ≤ -70°C for 4 years, ≤ -20°C for 6 months, and between 2°C and 8°C for 2 weeks. Aliquots can be stored between -20°C to -70°C for 3 months and between 2°C and 8°C for 1 week. For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Avoid repeated freeze/thaw cycles.
- Activity
- Cell-Vive™ GMP Recombinant Human Oncostatin M induces the proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 0.2 – 1.0 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human Oncostatin M is ≥ 1.7 x 107 IU/mg when compared against the WHO International Standard for human Oncostatin M (NIBSC code: 93/564).
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech-BioLegend@revvity.com.
- Disclaimer
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BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- Cytokine
- Function
- Proinflammatory mediator and keratinocyte activator, induces acute phase protein synthesis, hematopoiesis, and bone remodeling.
- Ligand/Receptor
- Receptor complex: type I and type II complexes are formed by oncostatin M receptor (OSMR), LIFR, and p130 (IL-6 signal transducer).
- Bioactivity
- Cell-Vive™ GMP Recombinant Human Oncostatin M induces the proliferation of human TF-1 cells.
- Cell Sources
- Activated monocytes, macrophages, T cells including skin-infiltrating T cells, and dendritic cells.
- Cell Targets
- Keratinocytes, fibroblasts, endothelia, hepatic cells, lung, hematopoietic cells, epithelial cells, and smooth muscle cells.
- Antigen References
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- Zarling JM, et al. 1986. Proc. Natl. Acad. Sci. USA. 83:9739.
- Rose TM and Bruce AG. 1991. Proc. Natl. Acad. Sci. USA. 88: 8641.
- Mosley B, et al. 1996. J. Biol. Chem. 271:32635.
- Dillon SR, et al. 2004. Nat. Immunol. 5:752.
- Boniface K, et al. 2007. J. Immunol. 178:4615.
- Beigel F, et al. 2014. PLoS One. 9:e93498.
- Schnittker D, et al. 2013. Mediators Inflamm. 2013:317503.
- Gene ID
- 5008 View all products for this Gene ID
- UniProt
- View information about Oncostatin M on UniProt.org
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