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- Other Names
- T-cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
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- Product Citations
- publications
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Cell-Vive™ GMP Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.5 ng/mL.
IL-2 was discovered through its function as a T cell growth factor (TCGF). It plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signaling pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by other cytokines. IL-2Rα is an IL-2–specific receptor, IL-2Rβ is shared with IL-15 and the γ chain that is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product DetailsBioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/µg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human IL-2 amino acid (Ala21-Thr153) (Accession: # P60568), was expressed in E.coli.
- Molecular Mass
- The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.41 kD. The DTT-reduced and non-reduced protein migrates at approximately 15.4 kD by SDS-PAGE.
-
N-terminal
Sequence Analysis - Met-Ala-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys & Ala-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.1 μm filtered protein solution is in 50 mM citric acid, 150 mM NaCl, pH 3.0.
- Endotoxin Level
- Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
- Residual Host Cell Protein Content
- ≤ 0.500 ng/μg by ELISA
- Concentration
- 25 µg, 100 µg & 1 mg sizes are bottled at 0.5 mg/mL
- Storage & Handling
- Unopened vials can be stored at ≤ -70°C for 4 years, ≤ -20°C for 6 months, and between 2°C and 8°C for 2 weeks. Aliquots can be stored between -20°C to -70°C for 3 months and between 2°C and 8°C for 1 week. For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Avoid repeated freeze/thaw cycles.
- Activity
- Cell-Vive™ GMP Recombinant Human IL-2 induces the proliferation of mouse HT- 2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.5 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human IL-2 is ≥ 2.4 x 106 IU/mg when compared against the WHO International Standard for human IL-2 (NIBSC code: 86/500).
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech-BioLegend@revvity.com.
- Disclaimer
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BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- Monomer
- Distribution
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T cells (CD4+ and CD8+), gamma δ T cells, B cells, dendritic cells, and eosinophils. Mainly activated T cells.
- Function
- Differentiation of Th1 and Th2 cells, inhibition of Th17 and Tfh cell differentiation, regulation of Treg differentiation, T cell proliferation, B cell proliferation, promotes CD8 and NK cell cytolytic activity.
- Interaction
- CD4 T cells, T reg cells, CD8 effector cells, NK cells, B cells
- Ligand/Receptor
- Trimeric receptor: IL-2Rα (CD25), IL-2Rβ (CD122), and γc (CD132) and dimeric receptor:IL-2Rβ (CD122) and γc (CD132)
- Bioactivity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose-dependent manner.
- Antigen References
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- Siegel JP, et al. 1987. Science. 238:75.
- Cote-Sierra J, et al. 2004. Proc Natl Acad Sci U S A. 101:3880.
- Laurence A, et al. 2007. Immunity. 26:371.
- Sakaguchi S, et al. 2008. Cell. 133:775.
- Liao W, et al. 2011. Nat Immunol. 12:551.
- Ballesteros-Tato A, et al. 2012. Immunity. 36:847.
- Liao W, et al. 2013. Immunity. 38:13.
- Read KA, et al. 2016. Exp Hematol. 44:799.
- Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
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