Loading...

Ultra-LEAF™ Purified anti-human SCF (KITLG) Antibody

Pricing & Availability
Clone
W24015B (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor, KIT-ligand, Kitl, KL-1, mast cell grow factor (MGF), steel factor(SF), FPH2, SHEP7
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
W24015B_ULEAF_SCF_Antibod_01272
Recombinant human CD117 (c-Kit)-Fc chimera (Cat. No. 597404; black circles) binds to immobilized recombinant human SCF (Cat. No. 573902). Ultra-LEAF™ Purified Anti-human SCF (KITLG) (clone W24015B, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified Rat IgG1, κ Isotype Control (Cat. No. 400431, gray triangles) has no inhibitory effect. This antibody blocks the binding of 50 ng/mL recombinant human CD117 (c-Kit)-Fc Chimera to 1.0 µg/mL of immobilized recombinant human SCF. The ND50 range to observe this effect is: 69 – 334 ng/mL. Main competitor ND50 = 733 ng/mL.
  • W24015B_ULEAF_SCF_Antibod_01272
    Recombinant human CD117 (c-Kit)-Fc chimera (Cat. No. 597404; black circles) binds to immobilized recombinant human SCF (Cat. No. 573902). Ultra-LEAF™ Purified Anti-human SCF (KITLG) (clone W24015B, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified Rat IgG1, κ Isotype Control (Cat. No. 400431, gray triangles) has no inhibitory effect. This antibody blocks the binding of 50 ng/mL recombinant human CD117 (c-Kit)-Fc Chimera to 1.0 µg/mL of immobilized recombinant human SCF. The ND50 range to observe this effect is: 69 – 334 ng/mL. Main competitor ND50 = 733 ng/mL.
Cat # Size Price Quantity Check Availability Save
648721 100 µg 240€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648722 1 mg 600€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Stem Cell Factor (SCF) was initially synthesized as a membrane-bound protein with 248 or 220 amino acids, depending on alternative splicing of exon 6. The 248 amino acid form contains a proteolytic cleavage site encoded by exon 6. It is cleaved from the cell to release an active soluble protein of 165 amino acid residues. Soluble SCF is glycosylated at both N-linked and O-linked sites. MMP-9 plays a physiological role in SCF release from the membrane. This action plays a significant role in differentiating and mobilizing stem and progenitor cells from the bone marrow. SCF increases the proliferation of myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF/c-kit interaction in mast cells results in mast cell degranulation with release of mediators, such as histamine and inflammatory cytokines and chemokines. Also, activation of c-kit in dendritic cells regulates T helper cell differentiation and allergic asthma. In addition, SCF plays an important role in revascularization of ischemic limbs. Ischemia induces plasma elevation of SCF and thrombopoietin (TPO). At lower levels, GM-CSF and erythropoietin (EPO). SCF and TPO induce the release of CXCL12 from platelets, thereby increasing CXCL12 levels in plasma. This results in an extensive mobilization of CXCR4+VEGFR1+ cells (hemangiocytes), accelerating revascularization of the ischemic limbs. SCF binds to its receptor kit that belongs to the type III tyrosine kinase family, whose members include receptors for M-CSF and PDGF.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human SCF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1 μg/mL immobilized recombinant human SCF (Cat. No. 573906) to its receptor recombinant human CD117 (c-Kit)-Fc Chimera (Cat. No. 597406) at 50 ng /mL. ND50 range: 69 – 334 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Distribution

Embryonic Stem Cells, Hematopoietic stem and progenitors

Interaction
Mast cells, natural killer cells, dendritic cells, eosinophils, epithelial cells, endothelial cells, melanocytes, germ cells, cholangiocytes, platelets, myeloid leukemia cells, and intestinal cells of Cajal
Ligand/Receptor
c-kit (CD117)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Lu HS, et al. 1996. J. Biol. Chem. 271:11309.
  2. Heissig B, et al. 2002. Cell. 109:625.
  3. Jin DK, et al. 2006. Nat. Med. 12:557.
  4. Krishnamoorthy N, et al. 2008. Nat. Med. 14:565.
  5. Ray P, et al. 2010. Ann. N. Y. Acad. Sci. 1183:104.
Gene ID
4254 View all products for this Gene ID
UniProt
View information about SCF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/27/2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account