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- Clone
- W6D3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 285604 | 50 µg | 316€ | ||||
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- WERI-RB-1 retinoblastoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3720747 (BioLegend Cat. No. 285604)
Antigen Details
- Structure
- Poly-N-acetyllactosamine
- Distribution
-
Neutrophils, eosinophils, monocytes
- Function
- Adhesion
- Cell Type
- Embryonic Stem Cells, Eosinophils, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks SC, et al. 1990. Biochem. J. 268:275.
- Gene ID
- NA
Related Pages & Pathways
Pathways
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
- What if my requested antibody-dye combination doesn't perform as expected?
-
Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.
Other Formats
View All CD15 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 785™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
PE/Dazzle™ 594 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 P... -
Brilliant Violet 421™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
APC/Fire™ 750 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
FITC anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Pacific Blue™ anti-human CD15 (SSEA-1)
-
TotalSeq™-A0392 anti-human CD15 (SSEA-1)
-
Brilliant Violet 711™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
APC/Cyanine7 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
TotalSeq™-B0392 anti-human CD15 (SSEA-1)
-
TotalSeq™-C0392 anti-human CD15 (SSEA-1)
-
PE anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Purified anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with purified W6... -
FITC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 FITC -
PE anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE -
APC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 APC -
Alexa Fluor® 488 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa ... -
Alexa Fluor® 647 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa ... -
PE/Cyanine5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE/Cya... -
PerCP anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PerCP -
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Pacific Blue™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Pacifi... -
Alexa Fluor® 700 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 510™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
PE/Cyanine7 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 605™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 650™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)
Human PBMCs (blue) and RBC-lysed human whole blood cells (re... -
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody
Human peripheral blood granulocytes were stained with anti-h... -
TotalSeq™-D0392 anti-human CD15 (SSEA-1)
-
GMP FITC anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Spark YG™ 593 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with anti-h... -
GMP PE anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
GMP Pacific Blue™ anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Spark Violet™ 500 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with anti-h... -
Spark UV™ 387 anti-human CD15 (SSEA-1)
Human peripheral blood leukocytes were stained with anti-hum... -
PE/Cyanine7 anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Spark YG™ 581 anti-human CD15 (SSEA-1) (Flexi-Fluor™)
-
Spark NIR™ 685 anti-human CD15 (SSEA-1) (Flexi-Fluor™)
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