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Purified anti-SMC3 Antibody

Pricing & Availability
Clone
W23212B (See other available formats)
Regulatory Status
RUO
Other Names
Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan (Bamacan), BAM, BMH, Chondroitin sulfate proteoglycan 6, CSPG6, Chromosome-associated polypeptide, hCAP
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W23212B_PURE_SMC3_WB_062625
Whole cell extracts (15 µg total protein per lane) from indicated cell lines and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SMC3 (clone W23212B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23212B_PURE_SMC3_WB_062625
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SMC3 (clone W23212B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23212B_PURE_SMC3_ICC_062625
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then either stained with Purified Rat IgG2b, κ Isotype Control (Cat. No. 400602) (Panel A) or Purified anti-SMC3 (clone W23212B) (Panel B), followed by incubation with Alexa Fluor® 647 Goat anti-rat (Cat. No. 400602) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • W23212B_PURE_SMC3_WB_2_062625
    Whole cell extracts (15 μg total protein per lane) from NIH/3T3 cell and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SMC3 (clone W23212B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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648805 25 µg 136€
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648806 100 µg 360€
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Description

SMC3 (Structural Maintenance of Chromosomes 3) is a core component of the cohesin complex, a group of proteins essential for chromosome cohesion, segregation, DNA repair, and transcriptional regulation in eukaryotic cells. SMC3 belongs to the Structural Maintenance of Chromosomes (SMC) family of ATPases, which includes other members such as SMC1, SMC2, SMC4, SMC5, and SMC6. Together with SMC1A, RAD21 (SCC1), and STAG proteins (SA1/SA2), SMC3 forms the ring-shaped cohesin complex that topologically encircles sister chromatids, holding them together after DNA replication until their separation during mitosis or meiosis. SMC3 and the broader cohesin complex are involved in regulating gene expression through chromatin architecture remodeling. Cohesin interacts with CTCF (CCCTC-binding factor), a key architectural protein, to form chromatin loops and topologically associating domains (TADs). These structures bring enhancers and promoters into close proximity, modulating transcriptional programs necessary for development and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein fragment of human SMC3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

RRID
AB_3717174 (BioLegend Cat. No. 648805)
AB_3717174 (BioLegend Cat. No. 648806)

Antigen Details

Structure
SMC3 is a 1217 amino acid protein with a predicted molecular weight of 141.5 kD.
Distribution

Located in nucleus

Function
Forms the ring-shaped cohesin complex
Interaction
Forms a heterodimer with SMC1A or SMC1B in cohesin complexes; interacts with RAD21 and STAG proteins
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Deardorff MA, et al. 2007. Am. J. Hum. Genet. 80:485-94.
  2. Beckouët F, et al. 2010. Mol. Cell. 39:689-99.
  3. Gil-Rodríguez MC, et al. 2015. Hum. Mutat. 36:454-62.
  4. Wang D, et al. 2018. Biomed. Pharmacother.104:788-797.
Gene ID
9126 View all products for this Gene ID
UniProt
View information about SMC3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/26/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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