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Purified anti-mouse ICAM-1 Antibody

Pricing & Availability
Clone
W21055I (See other available formats)
Regulatory Status
RUO
Other Names
CD54, Ly-47
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21055I_PURE_ICAM-1_ELISA_092425
Direct ELISA showing purified anti-mouse ICAM-1 (clone W21055I) bound plate immobilized recombinant mouse ICAM-1 (Cat Nos. 553004 and 553006). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-rat IgG (Cat No. 405405). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
  • W21055I_PURE_ICAM-1_ELISA_092425
    Direct ELISA showing purified anti-mouse ICAM-1 (clone W21055I) bound plate immobilized recombinant mouse ICAM-1 (Cat Nos. 553004 and 553006). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-rat IgG (Cat No. 405405). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
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612157 100 µg 280€
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Description

ICAM-1, also known as CD54 and Ly-47, is a 90 kD immunoglobulin superfamily member. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse ICAM-1 (carrier-free)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W21055I) could function as a capture antibody when paired with biotin anti-mouse ICAM-1 (Cat No. TBD) (Clone W21055A) using Recombinant mouse ICAM-1 (carrier-free) protein (Cat. Nos. 553004 and 553006) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3720584 (BioLegend Cat. No. 612157)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Springer TA. 1990. Nature. 346:425-34.
  3. Rothlein R, et al. 1986. J Immunol. 137:1270.
Gene ID
15894 View all products for this Gene ID
UniProt
View information about ICAM-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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