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Purified anti-MOG Antibody

Pricing & Availability
Clone
A24108A (See other available formats)
Regulatory Status
RUO
Other Names
Myelin Oligodendrocyte Glycoprotein, MOGIG2, BTN6, BTNL11, NRCLP7
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A24108A_PURE_MOG_IHC-P_092925
IHC staining with Purified anti-MOG (clone A24108A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-MOG (clone A24108A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale bar: 50 µm
  • A. A24108A_PURE_MOG_IHC-P_092925
    IHC staining with Purified anti-MOG (clone A24108A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-MOG (clone A24108A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale bar: 50 µm
  • B. A24108A_PURE_MOG_ICC_092925
    CHO cells non-transfected (negative expression, panel A) or transiently transfected (~20% positive expression, panel B) with an expression vector encoding human MOG were fixed and permeabilized with ice-cold methanol and then blocked with 5% FBS. Cells were then stained with Purified anti-MOG (clone A24108A), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. A24108A_PURE_MOG_WB_092925
    Whole cell extracts (15 µg total protein per lane) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-MOG (clone A24108A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • D. A24108A_PURE_MOG_ICFC_092925
    CHO cells non-transfected (negative expression, panel A) or transiently transfected (~20% positive expression, panel B) with an expression vector encoding human MOG were analyzed for flow cytometric analysis. The cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer set (Cat. No. 426803) and then intracellular stained with Purified anti-MOG (clone A24108A), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
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648880 25 µg 156€
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648881 100 µg 392€
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Description

Myelin oligodendrocyte glycoprotein (MOG) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. This heavily N-glycosylated protein features a single extracellular immunoglobulin-like domain, a transmembrane region, and a short intracellular tail. MOG is expressed on the surface of oligodendrocytes in the outermost layer of myelin and is highly conserved across species. MOG plays a critical role in the completion and maintenance of the myelin sheath, possibly acting as a cell adhesion molecule that contributes to myelin integrity and immune regulation. MOG is notably implicated in a group of autoimmune demyelinating disorders collectively known as MOG antibody-associated disease (MOGAD). In these conditions, the immune system produces antibodies targeting MOG, leading to inflammation and damage in the optic nerves, spinal cord, and brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MOG protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or True-Phos™ Perm Buffer (Cat. No. 425401).

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3720623 (BioLegend Cat. No. 648880)
AB_3720623 (BioLegend Cat. No. 648881)

Antigen Details

Structure
MOG consists of 247 amino acids and has a molecular weight of approximately 28.2 kDa
Interaction
• MOG acts as a receptor for rubella virus E2 Glycoprotein
• ARL6IP5
Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Cell Structure, Immunology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Salama S, et al. 2019. Mult Scler. 25(11):1427.
  2. Moseley CE, et al. 2024. Neurol Neuroimmunol Neuroinflamm. 11(5):e200275.
  3. Wynford TR, et al. 2019. J Neurol. 266(5):1280.
Gene ID
4340 View all products for this Gene ID
UniProt
View information about MOG on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All MOG Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-MOG A24108A IHC-P,ICC,ICFC,WB
Go To Top Version: 1    Revision Date: 09/29/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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