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Purified anti-Mcl-1 Antibody

Pricing & Availability
Clone
W23229E (See other available formats)
Regulatory Status
RUO
Other Names
Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT/mcl1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23229E_PURE_MCL-1_WB_082225
Indicated cell lysates (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-MCL-1 antibody (clone W23229E) for overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23229E_PURE_MCL-1_WB_082225
    Indicated cell lysates (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-MCL-1 antibody (clone W23229E) for overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23229E_PURE_MCL-1_ICC_082225
    SR cells (high-expressing cell line, panel B) and Jurkat cells (low-expressing cell line, panel A) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Mcl-1 (clone W23229E) and Purified mouse anti-PDHA1 (clone 3H2-F8-B5, Cat. No. 610951), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) and Alexa Fluor® 488 Goat anti-mouse IgG (Cat. No. 405319) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
648852 25 µg 120€
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648853 100 µg 300€
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Description

Mcl-1 is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. Mcl-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, Mcl-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life. Phosphorylation of at T92 and T163 by ERK inhibits Mcl-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of Mcl-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein comprising a portion of the N terminus of human Mcl-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only
Fixation/permeabilization with Fixation buffer followed by 0.5 % Triton X-100 is not recommended due to dim signal.

RRID
AB_3720577 (BioLegend Cat. No. 648852)
AB_3720577 (BioLegend Cat. No. 648853)

Antigen Details

Structure
Mcl-1 is a 350 amino acid protein with a predicted molecular weight of 37.3 kD.
Distribution

Tissue distribution: widely expressed
Cellular distribution: primarily mitochondria (outer membrane), also nucleus, cytosol and endoplasmic reticulum

Function
Anti-apoptotic/pro-survival protein
Interaction
Mcl-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References
  1. Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA. 90:3516.
  2. Zhou P, et al. 1997. Blood. 89:630.
  3. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883.
  4. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486.
  5. Moulding D, et al. 1998. Blood. 92:2495.
  6. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740.
  7. Opferman JT, et al. 2003. Nature. 426:671.
Gene ID
4170 View all products for this Gene ID
UniProt
View information about Mcl-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/22/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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