Loading...

Purified anti-LMO2 Antibody

Pricing & Availability
Clone
W23203A (See other available formats)
Regulatory Status
RUO
Other Names
RBTN2, RBTNL1, RHOM2, TTG2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A. W23203A_PURE_LMO2_WB_082125
Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-LMO2 (clone W23203A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23203A_PURE_LMO2_WB_082125
    Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-LMO2 (clone W23203A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23203A_PURE_LMO2_ICC_082125
    Jurkat cells (negative control, top panels) or TF-1 cells (positive control, bottom panels) were fixed and permeabilized with PFA and ice-cold methanol, and then intracellularly stained with Purified anti-LMO2 (clone W23203A) (right panels) or Purified Rat IgG2a Isotype Control (left panels) (Cat. No. 400502) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat No. 405416). Cells were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • C. W23203A_PURE_LMO2_ICFC_082125
    TF-1 cells (positive cell line, filled histogram) and Jurkat cells (negative cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-LMO2 (clone W23203A), or Purified Rat IgG2a, κ Isotype Control (dashed histogram, representative for both cell lines) (Cat. No. 400502) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
Cat # Size Price Quantity Check Availability Save
648846 25 µg 140€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648847 100 µg 360€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

LMO2 (LIM Domain Only 2) is a cysteine-rich protein containing two LIM domains, playing a pivotal role in hematopoietic development. It functions primarily as a transcriptional regulator, forming multiprotein complexes with other transcription factors such as TAL1/SCL and LDB1 to control gene expression critical for erythropoiesis and the maintenance of hematopoietic stem and progenitor cells. LMO2 is tightly regulated at the transcriptional level, with its expression modulated by various enhancers and transcription factors, including AML1a and SP1. Dysregulation of LMO2, particularly its ectopic expression in T cells, is strongly associated with T-cell acute lymphoblastic leukemia (T-ALL), where chromosomal translocations involving the LMO2 locus led to aberrant activation. Additionally, LMO2 has been implicated in other hematologic malignancies and may act as a proto-oncogene, highlighting its dual role in normal development and disease pathogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Fragment of Human LMO2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401), or True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401). We do not recommend Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

For use of this antibody in immunocytochemistry (ICC), we recommend fixation and permeabilization using either:
-PFA then ice cold methanol
-PFA then Triton X-100

Antigen Details

Structure
LMO2 is composed of 158 amino acids and has a predicted molecular weight of 18 kDa.
Distribution

Nucleus

Interaction
• LDB1 (LIM domain-binding protein 1)
• TAL1/SCL (T-cell acute lymphocytic leukemia protein 1)
• GATA1 and GATA2
• E2A and HEB
• Lyl1 and ETO2
• SP1
• ELK1, NFATc1, and LEF1
Cell Type
B cells, Hematopoietic stem and progenitors, Leukemia, Lymphocytes, Macrophages, Megakaryocytes, Monocytes
Biology Area
Adaptive Immunity, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Immuno-Oncology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Wang LL, et al. 2023. Cell Death Dis. 14(8):518
  2. Lossos C, et al. 2014. Br J Haematol. 165(5):640.
  3. Morishima T, et al. 2019. Blood. 134(14):1159.
  4. Latchmansingh KA, et al. 2022. Mod Pathol. 35(9):1220.
Gene ID
4005 View all products for this Gene ID
UniProt
View information about LMO2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/21/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account