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Purified anti-CTBP2 Antibody

Pricing & Availability
Clone
W23149C (See other available formats)
Regulatory Status
RUO
Other Names
C-Terminal Binding Protein 2, CtBP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23149C_PURE_CTBP2_WB_072525
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the Purified anti-CTBP2 (clone W23149C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23149C_PURE_CTBP2_WB_072525
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the Purified anti-CTBP2 (clone W23149C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23149C_PURE_CTBP2_ICC_072525
    The SH-SY5Y cells (high expression) and K562 cells (low expression) were fixed with Fixation buffer (Cat. No. 420801) for 10 minutes, permeabilized with Triton and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with Purified Rat IgG2a, κ Isotype Control Antibody (Cat. No. 400502) (panels A, D) or stained with Purified anti-CTBP2 (clone W23149C) (panels B, E), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (merged in panels C, F). The image was captured with a 40X objective. Scale bar: 25 µm
Cat # Size Price Quantity Check Availability Save
648834 25 µg 128€
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648835 100 µg 324€
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Description

C-terminal-binding protein 2, also known as CTBP2, is a transcriptional co‑repressor, originally discovered to bind to the carboxy-terminus of the human adenovirus E1A protein. CTBP family proteins are encoded by 2 paralogous genes, CTBP1 and CTBP2. CTBPs promote cancer cell migration, apoptosis antagonists, and tumor suppressor genes repressors. CTBP2 has been previously identified as being capable of directing cell migration by repressing PTEN expression and stimulating PI3K activity, which leads to Rac-dependent migration in some cancers. However, another study revealed that inhibiting CTBP2 enhanced stemness and the occurrence of primary ovarian carcinoma, while higher levels of CTBP2 expression correlate with improved overall survival (OS). These observations suggest that CTBP2 has two biological roles in influencing by different partner genes and downstream regulations. Furthermore, the investigations discovered that CTBP2 has a significant correlation between cell metabolism and the etiology of obesity in people.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CTBP2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

The clone cross-reacts with mouse in western blot (WB).

RRID
AB_3720545 (BioLegend Cat. No. 648834)
AB_3720545 (BioLegend Cat. No. 648835)

Antigen Details

Structure
CTBP2 is a 445 amino acid protein with a predicted molecular weight of 49 kD.
Distribution

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. Found in nucleus and synapse.

Function
Transcription regulators
Interaction
HIPK2, ZNF217, PRDM16, PNN, NRIP1, WIZ and MCRIP1.
Cell Type
Astrocytes, Microglia, Neurons
Biology Area
Cardiovascular Biology, Cell Biology, Neuroscience
Antigen References
  1. Chinnadurai G. 2009. Cancer Res. 69:731.
  2. Boyd JM, et al. 1993. EMBO J. 12:469.
  3. Katsanis, N. and Fisher, E.M. 1998. Genomics. 47:294.
  4. Paliwal S, et al. 2007. Cancer Res. 67:9322.
  5. Tracy-X C, et al. 2013. Immunity. 39:611.
  6. Sekiya M, et al. 2024. J Atheroscler Thromb. 31:109.
Gene ID
1488 View all products for this Gene ID
UniProt
View information about CTBP2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/25/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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