Loading...

Alexa Fluor® 647 anti-mouse Granzyme B Antibody

Pricing & Availability
Clone
S23006A (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Rat IgG1, λ
Ave. Rating
Submit a Review
Product Citations
publications
S23006A_A647_GranzymeB_ICFC_101025
C57BL/6 mouse splenocytes were stimulated with plate-bound Ultra-LEAF™ purified anti-mouse TCR β chain (Cat. No. 109254) for 2 days, and surface stained with anti-mouse CD8a (clone 53-6.7) PE. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-mouse Granzyme B (clone S23006A) Alexa Fluor® 647 (left) or rat IgG1, λ Alexa Fluor® 647 isotype control (right).
  • S23006A_A647_GranzymeB_ICFC_101025
    C57BL/6 mouse splenocytes were stimulated with plate-bound Ultra-LEAF™ purified anti-mouse TCR β chain (Cat. No. 109254) for 2 days, and surface stained with anti-mouse CD8a (clone 53-6.7) PE. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-mouse Granzyme B (clone S23006A) Alexa Fluor® 647 (left) or rat IgG1, λ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
168805 25 µg 148€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
168806 100 µg 333€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Granzyme B, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1, is a 32 kD serine protease. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clones S23006A and GB11 partially block each other.

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells

Function
Induction of cell death and apoptosis
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
Neutrophils, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
  2. Griffiths GM. and S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
  3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
  4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID
14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/10/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account