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Biotin anti-human YKL-40 (CHI3L1) Antibody

Pricing & Availability
Clone
A19120F (See other available formats)
Regulatory Status
RUO
Other Names
Chitnase-3-like protein 1, CHIL3L1, YKL-40, Cartilage glycoprotein 39, CGP-39, GP-39
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A19120F_Biotin_YKL-40_Elisa_092425
Direct ELISA showing biotin anti-human YKL-40 (CHI3L1) (clone A19120F) bound to plate-immobilized recombinant human YKL-40 (CHI3L1). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat No. 405103). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A19120F_Biotin_YKL-40_Elisa_092425
    Direct ELISA showing biotin anti-human YKL-40 (CHI3L1) (clone A19120F) bound to plate-immobilized recombinant human YKL-40 (CHI3L1). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat No. 405103). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450 nm.
Cat # Size Price Quantity Check Availability Save
612155 50 µg NOK2929
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Description

YKL-40, also known as CHI3L1 (Chitinase-3-like protein 1) or cartilage glycoprotein-39 (GP-39) is secreted by chondrocytes, synoviocytes, endothelial cells, smooth muscle cells and immune cells. YKL-40 plays a major role in different physiological processes including cell proliferation, tissue remodeling, angiogenesis, and apoptosis. Elevated serum levels of YKL-40 are linked to the pathogenesis and prognosis of several inflammatory reactions and autoimmune diseases, such as cancer, rheumatoid arthritis, liver cirrhosis, diabetes, and inflammatory bowel disease. Higher YKL-40 levels in cerebral spinal fluid are a known biomarker of brain inflammation and have a potential prognostic utility as a disease-specific marker in neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant CHI3L1 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A19120F) could function as a detection antibody when paired with purified anti-human YKL-40 (CHI3L1) antibody (Cat No. TBD) (clone W19120B) as the capture antibody and using Recombinant Human Chitnase 3-like 1 (carrier-free) (Cat No. 56802 and 568706) protein as the assay standard. For assay development flexibility purposes, this product is provided as a biotin format.

RRID
AB_3720582 (BioLegend Cat. No. 612155)

Antigen Details

Structure
Monomer
Distribution

Dendritic cells, Macrophages

Interaction
Macrophages, Neutrophils, T cells
Ligand/Receptor
IL-13Rα2, Syndecan-1
Cell Type
Endothelial cells, Granulocytes, Leukemia, Macrophages, Neurons
Biology Area
Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability
Molecular Family
Enzymes and Regulators
Gene ID
1116 View all products for this Gene ID
UniProt
View information about YKL-40 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 09.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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