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Purified anti-FABP2 Antibody

Pricing & Availability
Clone
W24079A (See other available formats)
Regulatory Status
RUO
Other Names
Fatty acid-binding protein 2, Fatty acid-binding protein, intestinal, FABPI, I-FABP
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
A. W24079A_PURE_FABP2_IHC-P_091525
IHC staining with Purified anti-FABP2 (clone W24079A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with Purified anti-FABP2 (clone W24079A) (panel A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel B). Scale bar: 50 µm
  • A. W24079A_PURE_FABP2_IHC-P_091525
    IHC staining with Purified anti-FABP2 (clone W24079A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with Purified anti-FABP2 (clone W24079A) (panel A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel B). Scale bar: 50 µm
  • B. W24079A_PURE_FABP2_WB_091525
    Whole cell extracts (15 µg total protein per lane) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-FABP2 (clone W24079A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
648872 25 µg NOK1155
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648873 100 µg NOK3177
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Description

Fatty acid-binding protein 2 (FABP2), also known as intestinal fatty acid-binding protein (I-FABP), is a small intracellular lipid-binding protein primarily expressed in the enterocytes of the small intestine. FABP2 belongs to the family of fatty acid-binding proteins that play crucial roles in lipid metabolism by binding long-chain fatty acids and other hydrophobic ligands. FABP2 is involved in the uptake, transport, and intracellular trafficking of dietary fatty acids from the intestinal lumen to metabolic or storage pathways, acting as a lipid chaperone. It contributes significantly to the regulation of lipid absorption and energy homeostasis. A polymorphism in FABP2, Ala54Thr (rs1799883), results in an amino acid substitution at position 54 and has been extensively studied for its metabolic implications. This variant is linked to several metabolic disorders, including insulin resistance, type 2 diabetes mellitus (T2DM), dyslipidemia, and obesity. In cardiovascular disease, FABP2 variants and expression levels are associated with altered lipid profiles and atherogenic risk, likely due to disrupted lipid transport from the gut to the circulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human FABP2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

RRID
AB_3720601 (BioLegend Cat. No. 648872)
AB_3720601 (BioLegend Cat. No. 648873)

Antigen Details

Structure
FABP2 is a 132 amino acid protein with a predicted molecular weight of 15.2 kD.
Distribution

Highly expressed in small intestine, with the highest expression levels in the jejunum. Subcellular localization is in the cytoplasm.

Biology Area
Cardiovascular Biology
Molecular Family
Apolipoproteins
Antigen References
  1. Albala C, et al. 2004. Obes. Res. 12:340-5.
  2. Tsai IT, et al. 2020. Int J Med. Sci. 17:2338-2345.
  3. Sekimori T, et al. 2024. J Integr Neurosci. 23:44.
Gene ID
2169 View all products for this Gene ID
UniProt
View information about FABP2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09.15.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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