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PE/Fire™ 744 anti-human CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
MIH3 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH3_PE-Fire744_CD274_Antibody_1_081825
PHA-activated (3 days) human peripheral blood lymphocytes were stained with anti-human CD3 (clone SK7) APC and anti-human CD274 (B7-H1, PD-L1) (clone MIH3) PE/Fire™ 744 (left) or mouse IgG1, κ PE/Fire™ 744 isotype control (right).
  • MIH3_PE-Fire744_CD274_Antibody_1_081825
    PHA-activated (3 days) human peripheral blood lymphocytes were stained with anti-human CD3 (clone SK7) APC and anti-human CD274 (B7-H1, PD-L1) (clone MIH3) PE/Fire™ 744 (left) or mouse IgG1, κ PE/Fire™ 744 isotype control (right).
Compare all formats See PE/Fire™ 744 spectral data
Cat # Size Price Quantity Check Availability Save
374521 25 tests NOK1609
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374522 100 tests NOK3713
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Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3720565 (BioLegend Cat. No. 374521)
AB_3720565 (BioLegend Cat. No. 374522)

Related FAQs

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Go To Top Version: 1    Revision Date: 08.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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