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Purified anti-human HMGB-1 Antibody

Pricing & Availability
Clone
W23075C (See other available formats)
Regulatory Status
RUO
Other Names
HMG-1, amphoterin, chromosomal protein non histone HMG1, HMGB-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W23075C_PURE_HMGB-1_ELISA_092425
Direct ELISA showing purified anti-human HMGB-1 (clone W23075C) bound plate immobilized recombinant human HMGB-1 (Cat No. 557804). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-rat IgG (Cat No. 405405). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
  • W23075C_PURE_HMGB-1_ELISA_092425
    Direct ELISA showing purified anti-human HMGB-1 (clone W23075C) bound plate immobilized recombinant human HMGB-1 (Cat No. 557804). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-rat IgG (Cat No. 405405). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
Cat # Size Price Quantity Save
612154 100 µg ¥82,500
Description

High mobility group box 1 (HMGB-1) is a protein highly conserved across species that is present in the nuclei (chromatin associated) and cytoplasm of all cells. In the cytoplasm, HMGB-1 is a critical regulator of autophagy, enhances cell survival, and limits apoptosis. It also acts as a chaperone that reduces protein aggregation caused by heat or chemical stress. HMGB-1 is released to the extracellular milieu by inflammatory cells and by necrotic and apoptotic cells. Once released, it functions as an inflammatory cytokine and it is secreted by macrophages and monocytes as a late response to LPS, TNF-α, IL-1β, or IFN-γ. Interestingly, the inflammatory activity of extracellular HMGB-1 is dependent upon its redox state. The protein possesses three cysteine residues: C23, C45, and C106. In its reduced state, HMGB-1, (-SH), has chemotactic activity; whereas HMGB-1, with a disulfide bond between C23 and C45, induces cytokine production and the activation of NF-κB. The fully oxidized form has no immune function, losing its proinflammatory effect and the caspase-dependent apoptotic cell death activation function. HMGB-1 interacts with several molecules, such as IL-1β, RNA, DNA, LPS, lipoteichoic acid (LTA), and CXCL12. Thus, HMGB-1 displays greater proinflammatory activity through cytokine binding, and the chemotactic property is mediated by CXCL12 binding. Extracellular HMGB-1 contributes to the pathogenesis of chronic inflammatory and autoimmune diseases, which includesepsis, rheumatoid arthritis, atherosclerosis, chronic kidney disease, systemic lupus erythematosus, and cancer. It has also been associated with chronic pain and delayed gastric ulcer healing in mice.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Recombinant HMGB-1 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W23075C) could function as a capture antibody when paired with biotin anti-human HMGB-1 antibody (Cat No. 612156) (Clone A18137B) as the detection antibody and using recombinant Human HMGB-1 (carrier-free) (Cat No. 557804) protein as the assay standard.

RRID
AB_3720581 (BioLegend Cat. No. 612154)

Antigen Details

Structure
Monomer
Distribution

Present in all cells' nuclei, released from a variety of cells including activated macrophages/monocytes, pituicytes, human PBMC, maturing dendritic cells, and murine erythroleukemia cells.

Function
HMGB-1 activates inflammatory responses, induces chemotactic responses, and the release of proinflammatory cytokines. It is induced by TNF-α, IL-1α, IFN-γ and LPS.
Interaction
T cells, macrophages, monocytes, dendritic cells, and neurons
Ligand/Receptor
RAGE, TLR2, TLR4, and TLR9
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Wang H, et al. 1999. Science. 285:248.
  2. Andersson U, et al. 2000. J. Exp. Med. 192:565.
  3. Scaffidi P, et al. 2002. Nature. 418:191.
  4. Tang D, et al. 2010. J. Cell Biol. 190:881.
  5. Venereau E, et al. 2012. J. Exp. Med. 209:1519.
  6. Min HJ, et al. 2013. J. Immunol. 190:1797.
Gene ID
3146 View all products for this Gene ID
UniProt
View information about HMGB-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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