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- Clone
- H5C6.Rec (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Thrombin-activated human peripheral blood platelets were stained with purified anti-human CD63 recombinant (clone H5C6.Rec) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
CD63, also referred to as LIMP, LAMP-3, gp55, TSPAN30, and melanoma-associated antigen ME491, is a 53 kD type III lysosomal-associated glycoprotein. As a member of the transmembrane 4 superfamily (TM4SF) of proteins known as tetraspanins, CD63 is broadly expressed on activated platelets as well as monocytes/macrophages, endothelial cells, fibroblasts, osteoclasts, and smooth muscle cells. It is implicated in platelet activation and is proposed to function as a transmembrane adaptor protein through interaction with other tetraspanins such as CD9 and CD81, and integrins including VLA-3 (α3β1) and VLA-6 (α6β1).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone H5C6.Rec can completely block the binding of clone H5C6 on target cells.
Antigen Details
- Structure
- 53 kD, type III lysosomal glycoprotein, tetraspan transmembrane superfamily (TM4SF) protein
- Distribution
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Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle
- Function
- Platelet activation
- Interaction
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Azorsa DO, et al. 1991. Blood 78:280.
- Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
- Hildreth JE, et al. 1991. Blood 77:121.
- Anzai N, et al. 2002. Blood 99:4413.
- Gene ID
- 967 View all products for this Gene ID
- UniProt
- View information about CD63 on UniProt.org
Related FAQs
Other Formats
View All CD63 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD63 Recombinant Antibody | H5C6.Rec | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD63 Recombinant Antibody
Thrombin-activated human peripheral blood platelets were sta...
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