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PE anti-ASC Antibody

Pricing & Availability
Clone
O93E9 (See other available formats)
Regulatory Status
RUO
Other Names
PYD and CARD domain-containing protein (PYCARD), Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain protein 5, Target of methylation-induced silencing (TMS1)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O93E9_PE_ASC_FC_071425
HL-60 cells (human leukemia cell line) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803), and then intracellularly stained with anti-ASC (clone O93E9) PE (filled histogram). Open histogram represents unstained cells.
  • O93E9_PE_ASC_FC_071425
    HL-60 cells (human leukemia cell line) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803), and then intracellularly stained with anti-ASC (clone O93E9) PE (filled histogram). Open histogram represents unstained cells.
See PE spectral data
Cat # Size Price Quantity Save
676503 25 tests ¥49,500
676504 100 tests ¥93,500
Description

Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1), PYD, and CARD domain containing protein (PYCARD), is a 21.6 kD pro-apoptotic protein that contains a N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm. However, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocates from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Also, the AIM2/ASC complex can act as a novel caspase-8 activation platform. Recent studes has shown that ASC reduces the phosphorylation of IKKα/β phosphorylation and inhibits NF-κB activity in primary melanoma. However, in metastatic melanoma through, it has the opposite function enhancing NF-κB activity and the secretion of IL-1β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human PYCARD (NP_037390) produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3717205 (BioLegend Cat. No. 676503)
AB_3717205 (BioLegend Cat. No. 676504)

Antigen Details

Structure
Full-length ASC (fASC) contains 195 amino acids wtih a predicted molecular weight of 21.6 kD. The sequence of isoform ASC-b is missing 93-111 a.a. The sequence of isoform ASC-c is missing 26-85 a.a.
Distribution

Cytoplasm. In resting monocytes and macrophages, the nucleus is the primary localization.

Function
ASC promotes caspase-mediated apoptosis. This pro-apoptotic activity is mediated predominantly through the activation of caspase-9. ASC is an integral component of the inflammasome. Within the inflammasome, ASC links caspase-1 and NOD-like receptors (NLR), which lead to the activation of caspase-1.
Ligand/Receptor
CIAS1/PYPAF1 and PYDC1.
Cell Type
Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References

1. Masumoto J, et al. 1999. J. Biol. Chem. 274:33835.
2. Conway KE, et al. 2000. Cancer Res. 60:6236.
3. Strong R, et al. 1991. Brain Res. 542:23.
4. Srinivasula SM, et al. 2002. J. Biol. Chem. 277:21119.
5. Ohtsuka T, et al. 2004. Nat. Cell Biol. 6:121.
6. Ippagunta SK, et al. 2010. J. Biol. Chem. 285:12454.
7. Liu W, et al. 2013. J. Invest Dermatol. 2:518-527.

Gene ID
29108 View all products for this Gene ID
UniProt
View information about ASC on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/14/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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