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Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
a-Poly4064_BV421_IgG_Antibody_FC_091725
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 (clone PK136) APC and, followed with (left) or without (right) donkey anti-rabbit IgG (clone Poly4064) Brilliant Violet 421™.
  • a-Poly4064_BV421_IgG_Antibody_FC_091725
    C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 (clone PK136) APC and, followed with (left) or without (right) donkey anti-rabbit IgG (clone Poly4064) Brilliant Violet 421™.
  • b-Poly4064_BV421_IgG_Antibody_IF_012213
    293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Save
406410 125 µL ¥44,880
Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Validated
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunocytochemistry, the suggested usage of this reagent is a 1:20-1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  2. Jin J, et al. 2023. Nat Aging. 3:600. PubMed
  3. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  4. Muto M, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016050496. PubMed
  5. Doehl JSP, et al. 2022. Front Immunol. 12:795554. PubMed
  6. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  7. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  8. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  9. Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed
  10. Madge P, et al. 2016. Sci Rep. 6:36012. PubMed
  11. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  12. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  13. Cao W, et al. 2016. Nat Commun. 7:11687. PubMed
  14. Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed
  15. Tsao LC, et al. 2022. JCI Insight. . PubMed
  16. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  17. Vogel R, Erez A, Altan-Bonnet G 2016. Nat Commun. 7: 12428. PubMed
  18. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  19. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  20. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  21. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  22. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  23. Yamasaki T, et al. 2018. J Virol. 92:e01458. PubMed
  24. Lamsoul I, et al. 2012. PLoS One. 7:e43798. PubMed
  25. Gao X, et al. 2021. Nature. 589:591. PubMed
  26. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  27. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  28. Bohlen CJ et al. 2017. Neuron. 94(4):759-773 . PubMed
  29. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  30. Becker L, et al. 2013. PLoS One. 8:e59452. PubMed
  31. Liddelow S, et al. 2017. Nature. 541:481-487. PubMed
  32. Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed
RRID
AB_10897810 (BioLegend Cat. No. 406410)

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 4    Revision Date: 09/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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