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Alexa Fluor® 647 anti-Olig2 Antibody

Pricing & Availability
Clone
W21023A (See other available formats)
Regulatory Status
RUO
Other Names
Oligodendrocyte transcription factor 2 (Olig2), Class B basic helix-loop-helix protein 1 (bHLHb1), Class E basic helix-loop-helix protein 19 (bHLHe19), Protein kinase C-binding protein 2 (PRKCBP2), RACK17, OLIGO2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W21023A_PURE_Olig2_ICFC_062025
HL-60 cells (positive cell line, filled histogram) and PC-3 cells (negative cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Olig2, or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (Cat. No. 400602).
  • a. W21023A_PURE_Olig2_ICFC_062025
    HL-60 cells (positive cell line, filled histogram) and PC-3 cells (negative cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-Olig2, or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (Cat. No. 400602).
  • b. W21023A_PURE_Olig2_ICC-1_062025
    Rat cortical stem cells were induced to differentiate with recombinant human FGF-basic (Cat. No. 788404) withdrawal and were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes. Then, the cells were permeabilized with 100% ice-cold methanol for 10 minutes, and subsequently blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-Olig2 (clone W21023A) (panel A, red). Cells were also stained with Alexa Fluor® 594 anti-Oligodendrocyte Marker O1 (panel B, pseudo color orange) and nuclei were counterstained with DAPI (Cat. No. 422801) (panel B, blue). The images were captured and merged by a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • c. W21023A_PURE_Olig2_ICC-2_062025
    U-87 MG cells were fixed with PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-Olig2 (clone W21023A) (panel A). Cells were also stained with Alexa Fluor® 488 anti-Tubulin-α (Cat. No. 627906) (panel B) and nuclei were counterstained with DAPI (Cat. No. 422801) (panel C). The images were captured and merged (panel D) by a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 μm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Save
615953 25 µg ¥38,500
615954 100 µg ¥96,800
Description

Olig2 is a transcription factor that plays a crucial role in the development and maintenance of the central nervous system. This transcription factor is a master regulator for oligodendrocyte cell specification. Olig2 activates the expression of myelin-associated genes in oligodendrocyte lineage cells, which are essential for proper neuronal function and transmission of nerve impulses. In addition to its role in oligodendrocyte differentiation and maturation, Olig2 also regulates the development and maintenance of motor neurons in the spinal cord and contributes to the proliferation and differentiation of neural stem cells. Dysregulation of Olig2 expression has been associated with several neurological disorders, including multiple sclerosis and gliomas, highlighting its importance in maintaining normal brain function. Being located on chromosome 21, dysregulation of Olig2 is also associated with Down Syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the N-terminus of mouse Olig2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
- Fixation Buffer (Cat. No. 420801) with 0.5 % Triton X-100
- Fixation Buffer (Cat. No. 420801) with 100% ice-cold methanol

For use in intracellular flow cytometry (ICFC), it is recommended to fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401).

RRID
AB_3720495 (BioLegend Cat. No. 615953)
AB_3720495 (BioLegend Cat. No. 615954)

Antigen Details

Structure
Olig2 is a 323 amino acid protein with a predicted molecular weight of 32.38 kD.
Distribution

Nucleus

Function
Transcription factor
Cell Type
Neural Stem Cells, Oligodendrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Antigen References
  1. Zhang K, et al. 2022. Nat Commun. 13:1423.
  2. Xu R, et al. 2019. Cell Stem Cell. 24:908-926.
  3. Sock E, Wegner M. 2021. Dev Neurobiol. 81:892-901. 
  4. Szu J, et al. 2021. Front Neurosci. 15:659601.
Gene ID
10215 View all products for this Gene ID
UniProt
View information about Olig2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/20/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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