Recombinant Mouse IL-27 (carrier-free)

Product Details

Source

Mouse IL-27 consists of mouse EBI3 (NM_015766, Tyr19-Pro228), and mouse p28 (NM_145636, Phe29-Ser234) joined via a linker, expressed in 293E cells with a 8His-tag in the C-terminus.

Molecular Mass

The total predicted molecular weight is 49 kD. The non-reduced and the DTT-reduced proteins migrate as one band at approximately 55 kD by SDS-PAGE.

Purity

> 95%, as determined by Coomassie stained SDS-PAGE.

Formulation

Lyophilized from 0.22 µm filtered protein solution in 20 mM HEPES, 300 mM NaCl, 2 mM CHAPS, 5% Trehalose, pH 7.2

Endotoxin Level

Less than 0.1 EU per µg of cytokine as determined by the LAL method.

Concentration

50 µg size lyophilized

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile DI water, pH 7.2. Before reconstitution, make sure sterile DI water and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile DI water) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at an ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all Recombinant mouse IL-27 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED₅₀ for this effect is 0.2 - 1 ng/mL the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.

Activity

Recombinant mouse IL-27 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED₅₀ for this effect is 0.2 – 1.4 ng/mL

Application

Bioassay

Application Notes

Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure

Dimer

Distribution

Produced by antigen-presenting cells, including macrophages and dendritic cells.

Function

IL-27 triggers rapid clonal expansion of antigen-specific naïve human and mouse CD4 T cell. IL-27 promotes the early Th1 differentiation through up-regulation of ICAM-1 and T-bet and suppresses the differentiation to Th2 and Th17. In addition, IL-27 suppresses the cytokine production by activated CD4+ T cells in vitro, and TNF-α and IL-12 production by macrophages. This suppressive mechanism is considered to be mediated through IL-10. IL-27 plays a dominant function together with TGF-β in generating IL-10-producing anti-inflammatory Tr1 cells. Additionally, IL-27 exerts IFN-like functions in liver cells. IL-27 is predominantly produced early after stimulation of APCs by TLR ligands or infectious agents.

Interaction

Activated T cells, resting NK cells, resting NKT cells, hepatoma cells, and hepatocytes.

Ligand/Receptor

IL-27Ra (also known as WSX-1 or TCCR) paired with gp130

Bioactivity

Recombinant mouse IL-27 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner.

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction

Molecular Family

Cytokines/Chemokines

Antigen References

1. Pflanz S, et al. 2002. Immunity. 16:779.
2. Owaki T, et al. 2005. J Immunol. 175:2191.
3. Batten M, et al. 2006. Nat Immunol. 7:929.
4. Villarino VA, et al. 2006. J Immunol. 176:237.
5. Stumhofer JS, et al. 2007. Nat Immunol. 8:1363.
6. Awasthi A, et al. 2007. Nat Immunol. 8:1380.
7. Pot C, et al. 2009. J Immunol. 183:797.
8. Bender H, et al. 2009. Hepatology. 50:585.

Gene ID

246779 View all products for this Gene ID

UniProt

View information about IL-27 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Certificate of Analysis