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Purified anti-KDM3B (JMJD1B) Antibody

Pricing & Availability
Clone
W20097B (See other available formats)
Regulatory Status
RUO
Other Names
Lysine-specific demethylase 3B, JmjCdomain-containing histone demethylation protein 2B, Jumonjidomain-containing protein 1B, Nuclear protein 5qNCA, [histoneH3]-dimethyl-L-lysine(9) demethylase 3B, C5orf7, JHDMB
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W20097B_PURE_KDM3B_1_111225
Whole cell extracts (15 µg total protein per lane) from HAP1 wildtype and HAP1 Knockout cells (HAP1 KDM3B Knockout cells, Horizon Cat. No. HZGHC001145c010) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-KDM3B (JMJD1B) (clone W20097B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W20097B_PURE_KDM3B_1_111225
    Whole cell extracts (15 µg total protein per lane) from HAP1 wildtype and HAP1 Knockout cells (HAP1 KDM3B Knockout cells, Horizon Cat. No. HZGHC001145c010) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-KDM3B (JMJD1B) (clone W20097B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W20097B_PURE_KDM3B_2_111225
    Whole cell extracts (250 µg total protein) prepared from HAP1 wild-type and HAP1 KDM3B knockout cells (Horizon Cat. No. HZGHC001145c010) were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl (Cat. No. 400502) or Purified anti-KDM3B (clone W20097B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a different antibody against a separate epitope of KDM3B. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
648896 25 µg 140€
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648897 100 µg 376€
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Description

Lysine demethylase 3B (KDM3B), also known as JHDM2B or JMJD1B, is a member of the Jumonji C (JmjC) domain-containing histone demethylase family, which participates in the epigenetic regulation of gene expression. KDM3B specifically catalyzes the demethylation of mono- and di-methylated lysine 9 on histone H3 (H3K9me1/2), a repressive chromatin mark, thereby promoting transcriptional activation. KDM3B interacts with other chromatin remodelers, transcription factors, and noncoding RNAs to regulate gene networks critical for normal physiology. Dysregulation of KDM3B has emerged as a contributing factor in the pathogenesis of multiple human diseases, such as cancer and neurodevelopmental disorders. In hematopoiesis, KDM3B functions as a regulator of gene expression programs essential for stem and progenitor cell maintenance. Loss-of-function mutations or deletions of KDM3B have been reported in myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and other hematologic malignancies. KDM3B deficiency leads to aberrant H3K9 methylation, resulting in transcriptional silencing of genes required for differentiation and genome stability. Overexpression of KDM3B has been observed in hepatocellular carcinoma and colorectal cancer, where it enhances glycolysis and angiogenesis through upregulation of HIF-1α target genes, thereby supporting tumor growth and survival.  KDM3B is implicated in neurodevelopmental and psychiatric disorders. Haploinsufficiency or mutations in the KDM3B gene have been associated with intellectual disability, developmental delay, and autism spectrum disorder. The underlying mechanisms are thought to involve disrupted chromatin states leading to impaired transcriptional programs governing neuronal differentiation, synapse formation, and plasticity.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 KDM3B KO cells (Horizon Cat. No. HZGHC001145c010).

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human KDM3B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
KDM3B is a 1761 amino acid protein with a predicted molecular weight of 191.6 kD.
Distribution

Nucleus

Function
Demethylate Lys-9 of histone H3 and play a central role in histone modification
Interaction
Lys-9 of histone H3
Biology Area
Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers
Antigen References
  1. Kim JY, et al. 2012. Mol. Cell Biol. 32:2917-2933.
  2. Li S, et al. 2018. Cell Rep. 23:389-403.
  3. Diets IJ, et al. 2019. Am. J. Hum. Genet. 104:758-766.
  4. Xu X, et al. 2018. Leuk. Lymphoma. 59:204-213.
Gene ID
51780 View all products for this Gene ID
UniProt
View information about KDM3B on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/12/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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