Purified anti-human CD227 (MUC-1) Antibody, CD227, DF3

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Clone: DF3 (See other available formats)
Regulatory Status: RUO
Other Names: Mucin 1, Episialin, MAM-6, CA 15-3, PEM, CD227, EMA
Isotype: Mouse IgG1, κ
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Product Citations: publications

DF3_PURE_CD227_IHC-P_1_040125 — IHC staining of purified anti-human CD227 (MUC-1) (clone DF3) on formalin-fixed, paraffin-embedded human breast carcinoma tissue. Following antigen retrieval using Tris-EDTA Buffer (Cat. No. 422704), formalin-fixed, paraffin-embedded human breast carcinoma tissues were incubated with 5 µg/mL of purified mouse IgG1, κ isotype control (panel A) or purified anti-human CD227 (MUC-1) (clone DF3) (panel B) overnight at 4°C. Tissues were then stained with anti-mouse IgG1 Alexa Fluor® 647 (red) (Cat. No. 406618). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 μm

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311203

100 µg

200€

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Description

Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers. In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed. The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens. It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex. MUC-1 can interact with cancer antigens such as Her2/neu. Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers. In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed. The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens. It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex. MUC-1 can interact with cancer antigens such as Her2/neu.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Membrane enriched fractions of human metastatic breast carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone DF3 recognizes the extracellular domain of the MUC1 protein, specifically the tandem repeat region.
RRID: AB_3698967 (BioLegend Cat. No. 311203)

Antigen Details

Structure: 500-1000 kD proteoglycan (glycosylated form)
Distribution: Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers
Interaction: Complexes with SOS1 and Grb2 in RAS signaling
Ligand/Receptor: ICAM-1 and Her2/neu (ErbB2)
Cell Type: Dendritic cells, Epithelial cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Yasui S, et al. 1994. Acta Derm Venereol. 74:98-100.

Ichige K, et al. 1995. Clin Cancer Res. 1:565-71.
Gene ID: 4582 View all products for this Gene ID
UniProt: View information about CD227 on UniProt.org

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Other Formats

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Description	Clone	Applications
Purified anti-human CD227 (MUC-1)	DF3	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/10/2025

For Research Use Only. Not for diagnostic or therapeutic use.

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