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Purified anti-EHF Antibody

Pricing & Availability
Clone
W22125A (See other available formats)
Regulatory Status
RUO
Other Names
EHF; Epithelium-specific ETS transcription factor 3; ESE-3; ESE3 transcription factor; ETS domain-containing transcription factor; ETS homologous factor.
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22125A_PURE_EHF_WB_62425
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-EHF (clone W22125A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22125A_PURE_EHF_WB_62425
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-EHF (clone W22125A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22125A_PURE_EHF_ICC_62425
    LNCaP cells with (EHF KD) and without (mock KD) transient transfection of EHF shRNA were fixed with fixation buffer (Cat. No. 420801) for 10 minutes, permeabilized with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with isotype control (Cat. No. 400602) (panels A and D) or stained with Purified anti-EHF (clone W22125A) (panels B and E), followed by incubation with Alexa Fluor 594 Goat anti-rat IgG (red, Cat. No. 405422) for 1 hour at room temperature. Then, cells were co-stained with Alexa Fluor® 488 anti-GFP Antibody (green, Cat. No. 338008) (merged in panels C and F) for 1 hour at room temperature to detect the knockdown reporter in transfected cells. Nuclei were counterstained with DAPI (Cat. No. 422801) (merged in panels C and F). The image was captured with a 40X objective. Scale bar: 25 µm
Cat # Size Price Quantity Check Availability Save
659151 25 µg 144€
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659152 100 µg 380€
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Description

ETS homologous factor (EHF), also known as epithelium-specific ETS transcription factor 3 (ESE-3), is a member of the ETS family of transcription factors expressed in various epithelial cell types, which participates in the oncogenesis of many malignant tumors through regulating the cell proliferation and differentiation. The genome-wide association study (GWAS) identified that genetic markers of lung disease severity in cystic fibrosis (CF) led to renewed interest in the potential importance of EHF in lung disease. Studies have revealed that EHF transcript variants such as EHF-LF and EHF-SF may have different impacts on epithelial cell differentiation and can promote or inhibit the epithelial mesenchymal transition (EMT) phenomenon. EHF also has been found in kidney, lung and somatotroph tumors and may serve as a prognostic biomarker. According to recent studies, EHF has been shown to inhibit pancreatic ductal adenocarcinoma (PDAC) metastasis through up-regulation of E-cadherin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human EHF protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For immunocytochemistry (ICC), we recommend 4% PFA fixation followed by permeabilization with Triton X-100 or ice-cold methanol.

This antibody (clone W22125A) is not suitable for IHC.

This antibody (clone W22125A) does not cross-react with mouse in western blot (WB).

RRID
AB_3717146 (BioLegend Cat. No. 659151)
AB_3717146 (BioLegend Cat. No. 659152)

Antigen Details

Structure
EHF is a 300 amino acid protein with a predicted molecular weight of 35 kD.
Distribution

Nucleus

Function
Transcriptional activator, regulating epithelial cell differentiation
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Transcription Factors
Molecular Family
Tumor Suppressors
Antigen References
  1. Fossum S.L, et al. 2017. J Biol Chem. 292:10938.
  2. Wright F. A, et al. 2011. Nat Genet. 43:539.
  3. Sakamoto K, et al. 2021. Oncogenesis. 10:26.
  4. Liu J, et al. 2019. J Exp Med. 216:656.
Gene ID
26298 View all products for this Gene ID
UniProt
View information about EHF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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