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Purified anti-Carbonic Anhydrase 2 (CA2) Antibody

Pricing & Availability
Clone
A18177A (See other available formats)
Regulatory Status
RUO
Other Names
CA2, CA-II, Carbonate dehydratase II, Carbonic anhydrase C, CAC, HEL-76
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
a. A18177A_PURE_CA2_WB_052825
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Carbonic Anhydrase 2 (CA2) (clone A18177A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. A18177A_PURE_CA2_WB_052825
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Carbonic Anhydrase 2 (CA2) (clone A18177A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. A18177A_PURE_CA2_IHC-P_052825
    IHC staining with Purified anti-Carbonic Anhydrase 2 (CA2) (clone A18177A) on formalin-fixed paraffin-embedded human colon tissue following antigen retrieval using Citrate buffer (Cat. No. 420902). The tissue was incubated without (panel A) and with (panel B) Purified anti-Carbonic Anhydrase 2 (CA2) (clone A18177A) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • C. A18177A_PURE_CA2_ICFC_052825
    THP-1 cells (positive cell line, filled histogram) and Jurkat cells (negative cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-Carbonic Anhydrase 2 (CA2) (clone A18177A), or Purified Mouse IgM, κ Isotype Control (Cat. No. 401602) (open histogram, dashed line, representative of both the positive and negative cell lines) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
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654451 25 µg 124€
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654452 100 µg 328€
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Description

Carbonic anhydrase 2 (CA2) is an enzyme that catalyzes the reversible hydration of carbon dioxide, playing a role in maintaining acid-base balance and facilitating CO2 transport in the blood. It is regulated by various factors, including the availability of zinc ions, which are essential for its catalytic activity. CA2 is involved in several physiological processes, such as respiration and pH regulation in tissues. Defects in CA2 can lead to diseases like osteopetrosis, a condition characterized by overly dense bones, and renal tubular acidosis, which affects the kidney ability to maintain acid-base balance. Additionally, CA2 deficiency can result in brain calcifications and developmental delays.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant Carbonic Anhydrase 2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). We do not recommend True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3717113 (BioLegend Cat. No. 654451)
AB_3717113 (BioLegend Cat. No. 654452)

Antigen Details

Structure
Carbonic anhydrase 2 (CA2) consists of 260 amino acids and has a molecular weight of approximately 29.2 kDa.
Distribution

Carbonic anhydrase 2 (CA2) is primarily located in the cytoplasm

Function
• Catalyzes CO2 Hydration: Converts carbon dioxide (CO2) to bicarbonate (HCO3-) and protons (H+), facilitating CO2 transport and maintaining acid-base balance.
• pH Regulation: Helps regulate pH in various tissues, including red blood cells, kidneys, and the gastrointestinal tract.
• Respiratory Function: Plays a crucial role in the rapid exchange of CO2 and oxygen in the lungs
• Bone Resorption: Involved in bone resorption by osteoclasts, essential for bone remodeling.
Interaction
• Band 3 (AE1): The erythrocyte anion exchanger involved in chloride/bicarbonate exchange.
• Sodium-Hydrogen Antiporter 1 (NHE1): A protein that helps regulate intracellular pH by exchanging sodium ions for hydrogen ions across the cell membrane.
Cell Type
Antigen-presenting cells, Erythrocytes, Macrophages, Monocytes, Osteoclasts
Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Ronca R, et al. 2024. Biochim Biophys Acta Rev Cancer. 1879::189120.
  2. Tsikas D. 2024. J Enzyme Inhib Med Chem. 39:2291336.
  3. Alkhayal Z, et al. 2023. Curr Issues Mol Biol. 45:1373-1386.
  4. Ciccone L, et al. 2021. Molecules. 26:6380.
Regulation
• Zinc Ion Availability: Requires zinc ions for its catalytic activity; zinc binding is crucial for enzyme function.
• Gene Expression: Regulated at the transcriptional level by various factors, including hormones and cellular pH.
• Inhibitors: Can be inhibited by specific drugs, such as acetazolamide, which are used to treat conditions like glaucoma and epilepsy
Gene ID
760 View all products for this Gene ID
UniProt
View information about Carbonic Anhydrase 2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/28/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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