PE anti-human CD50 (ICAM-3) Antibody, CD50, A22041E

Pricing & Availability

Clone: A22041E (See other available formats)
Regulatory Status: RUO
Other Names: ICAM-3
Isotype: Mouse IgG2b, κ
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Product Citations: publications

A22041E_PE_CD50_FC_091725 — Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone A22041E) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

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377117

25 tests

100€

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377118

100 tests

176€

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Description

CD50, also known as intercellular adhesion molecule 3 (ICAM-3), is a 120-140 kD type I membrane protein and member of the Ig superfamily. It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 provides costimulatory signals for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, α_L/β₂) integrin.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human ICAM3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3720617 (BioLegend Cat. No. 377117)
AB_3720617 (BioLegend Cat. No. 377118)

Antigen Details

Structure: Ig superfamily, type I membrane protein, 130 kD
Function: Adhesion, costimulation
Interaction: Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells
Ligand/Receptor: CD11a/CD18 (LFA-1), integrin α_L/β₂
Cell Type: B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Valent P, et al. 2001. Immunol. Rev. 179:74.
Gene ID: 3385 View all products for this Gene ID
UniProt: View information about CD50 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD50 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD50 (ICAM-3)	A22041E	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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