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- Regulatory Status
- RUO
- Other Names
- FAP, FAP-alpha, Fibroblast activation protein alpha, Integral membrane serine protease, Seprase, DPP4
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min. -
Stability testing for Biotinylated human FAP: Biotinylated human FAP was aliquoted in 16 mM Tris, 240 mM NaCl, 20% Glycerol, pH 7.5 at 0.2 mg/mL and one aliquot was kept at 4°C (Control) for 7 days and another aliquot was frozen and thawed four times (4x Freeze/Thaw) over a period of one week. After this procedure, samples were tested for their ability to cleave a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 768914 | 25 µg | 344€ | ||||
| 768916 | 100 µg | 812€ | ||||
Fibroblast activation protein (FAP) is a cell-surface transmembrane-anchored dimeric proteas. FAP expression is very low in adult organs but is upregulated by activated fibroblasts at the sites of tissue remodeling, including fibrosis, atherosclerosis, arthritis and tumors. FAP is broadly expressed on cancer associated fibroblasts (CAFs), which are a major component of the stromal microenvironment of carcinomas. FAP has been identified as a promising immunotherapy target antigen for epithelial carcinomas such as prostate, lung, pancreatic cancer and mesothelioma. It has been shown that by removing the fibrosis barrier, ‘FAP-Chimeric Antigen Receptor-Macrophages’ enhanced the penetration of gemcitabine and immune cells, improved PDAC (pancreatic ductal adenocarcinoma) sensitivity to chemotherapy and immunotherapy and significantly prolonged survival. FAP is not commonly expressed by blood vessels within healthy tissues or by normal endothelial cells (ECs) but can be strongly and consistently induced on ECs within the glioblastoma microenvironment. FAP directly cleaves CXCL12 to inactivate canonical wnt signaling and therefore plays a direct role in regulating osteogenic and adipogenic differentiation of mesenchymal progenitor cells.
Product DetailsProduct Details
- Source
- Human FAP, amino acids Leu26-Asp760 (Accession # Q12884), with a C-terminal 6His tag and Avi tag was expressed in CHO cells. Human FAP was site-specifically biotinylated by BirA.
- Molecular Mass
- The 761 amino acid recombinant protein has a predicted molecular mass of approximately 87.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 80-90 kD in SDS-PAGE.
-
N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 16 mM Tris, 240 mM NaCl, 20% Glycerol, pH 7.5
- Endotoxin Level
- Less than 0.1 EU per μg of protein as determined by the LAL method
- Concentration
- 25 µg and 100 µg at 1.0 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Adipose tissue and Cancer associated fibroblasts (CAFs)
- Function
- Tissue remodeling, fibrosis, wound healing, inflammation, tumor growth and metastasis
- Interaction
- Serpin F2 (alpha-2-antiplasmin); Gelatin (Type I collagen)
- Bioactivity
- Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min.
- Biology Area
- Cancer Biomarkers
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Ostermann E. et al. 2008. Clin Cancer Res.14:4584.
- Kakarla S. et al. 2013. Mol Ther. 21:1611.
- Gulati P. et al. 2018. Clin Cancer Res. 24:3981.
- Freedman JD. et al. 2018. Cancer Res. 78: 6852.
- Schuberth PC. et al. 2013. J Transl. Med. 11:187
- Garin‐Chesa P. et al. 1990. Proc Natl Acad Sci USA. 87:7235.
- Nurmik M. et al. 2020. Int J Cancer. 146:895.
- Wang W. et al. 2025. J Control Release. 384:113888
- Gene ID
- 2191 View all products for this Gene ID
- UniProt
- View information about FAP on UniProt.org
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