StarBright UltraViolet 740 anti-mouse CD4 antibody, CD4, RM4-5

Pricing & Availability

Clone: RM4-5 (See other available formats)
Regulatory Status: RUO
Other Names: L3T4, T4
Isotype: Rat IgG2a, κ
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Product Citations: publications

SBUV740_RM4-5_FC_BasicPanel_071425 — C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) PE and anti-mouse CD4 (clone RM4-5) StarBright UltraViolet 740 (left) or stained with anti-mouse CD3ε (clone 145-2C11) PE only (right).

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100588

30 µg

93€

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100587

150 μg

207€

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: 0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4⁺ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4⁺ cells¹, and immunohistochemistry of acetone-fixed frozen tissue sections^2,3,11 and paraffin-embedded sections¹¹. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Dendritic cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
Gene ID: 12504 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
PE/Cyanine7 anti-mouse CD4	RM4-5	FC
APC/Cyanine7 anti-mouse CD4	RM4-5	FC
APC/Fire™ 750 anti-mouse CD4	RM4-5	FC
TotalSeq™-A0001 anti-mouse CD4	RM4-5	PG
TotalSeq™-B0001 anti-mouse CD4	RM4-5	PG
TotalSeq™-C0001 anti-mouse CD4	RM4-5	PG
Ultra-LEAF™ Purified anti-mouse CD4	RM4-5	FC,CyTOF®,Depletion,Block,IHC
APC anti-mouse CD4	RM4-5	FC
Biotin anti-mouse CD4	RM4-5	FC
FITC anti-mouse CD4	RM4-5	FC
PE anti-mouse CD4	RM4-5	FC
PE/Cyanine5 anti-mouse CD4	RM4-5	FC
Purified anti-mouse CD4	RM4-5	FC,CyTOF®,IHC-F,IHC
Alexa Fluor® 647 anti-mouse CD4	RM4-5	FC,3D IHC,IHC-F
Alexa Fluor® 488 anti-mouse CD4	RM4-5	FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD4	RM4-5	FC
Alexa Fluor® 700 anti-mouse CD4	RM4-5	FC
PerCP anti-mouse CD4	RM4-5	FC
PerCP/Cyanine5.5 anti-mouse CD4	RM4-5	FC
Brilliant Violet 421™ anti-mouse CD4	RM4-5	FC,ICC,IHC-F
Brilliant Violet 570™ anti-mouse CD4	RM4-5	FC
Brilliant Violet 605™ anti-mouse CD4	RM4-5	FC
Brilliant Violet 650™ anti-mouse CD4	RM4-5	FC
Brilliant Violet 711™ anti-mouse CD4	RM4-5	FC
Brilliant Violet 785™ anti-mouse CD4	RM4-5	FC
Brilliant Violet 510™ anti-mouse CD4	RM4-5	FC
Purified anti-mouse CD4 (Maxpar® Ready)	RM4-5	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD4	RM4-5	FC
Spark Violet™ 423 anti-mouse CD4	RM4-5	FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™)	RM4-5	FC
APC/Fire™ 810 anti-mouse CD4	RM4-5	FC
PE/Fire™ 640 anti-mouse CD4 Antibody	RM4-5	FC
Brilliant Violet 750™ anti-mouse CD4 Antibody	RM4-5	FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™)	RM4-5	FC
StarBright UltraViolet 740 anti-mouse CD4	RM4-5	FC
Spark PLUS V450™ anti-mouse CD4	RM4-5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/06/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.