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- Regulatory Status
- RUO
- Other Names
- IL-1F1, IL-1 alpha, Hematopoietin-1, interleukin 1 alpha
- Ave. Rating
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- Product Citations
- publications
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Recombinant rat IL-1α induces proliferation of D10.G4.1 (mouse helper T lymphocyte) in a dose-dependent manner. ED50 of this effect is 3-30 pg/mL. -
Stability testing for recombinant rat IL-1α. Recombinant rat IL-1α was aliquoted in 50mM Tris, pH 8.0, at 0.2 mg/ml and one aliquot was kept at 4°C (Control) and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce proliferation of D10.G4.1 (mouse helper T-lymphocyte) in a dose-dependent manner. ED50 of this effect is 3-30 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 720204 | 25 µg | 304€ | ||||
| 720206 | 100 µg | 760€ | ||||
IL-1 is a pleiotropic pro-inflammatory cytokine that exhibits biological activities supporting its role in tumor growth and metastasis. IL-1α is usually expressed as a preprotein, which is secreted only after cleavage by the calpain family of proteases. Calpains cleave pre-IL-1α and release the N-terminal propiece-IL-1α and the C-terminal secreted IL-1α. Unlike IL-1β, which is biologically active only as a secreted mature molecule, membrane-associated IL-1α, propiece-IL-1α, and mature secreted IL-1α show distinct biological activities. The propiece-IL-1α has transforming activity whereas secreted IL-1α has paracrine and autocrine activities like IL-1β. IL-1α stimulates vascularization and proliferation of cancer-associated fibroblasts thus contributes to cancer progression. IL-1α is a potent proinflammatory cytokine that mediates inflammation, microbial invasion and tissue injury. It is expressed in various immune cells, including macrophages and monocytes. It also acts as a mediator of inflammatory responses, inducing inflammation via IL-1R1-dependent pathways upon release into the tumor microenvironment. This causes persistent inflammation and stimulates the production of other proinflammatory cytokines, such as IL-6, IL-8, and TNF-α. The persistent inflammatory microenvironment leads to the deterioration of the pathological condition. IL-1α-neutralizing antibodies are currently employed in the treatment of autoimmune diseases, such as rheumatoid arthritis, as they alleviate joint inflammation via modulation of IL-1α concentrations.
Product DetailsProduct Details
- Source
- Rat IL-1α amino acids Ser115-Ser270 (Accession # P16598) was expressed in E.coli
- Molecular Mass
- The 157 amino acid recombinant protein has a predicted molecular mass of approximately 17.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 17-25 kD in SDS-PAGE.
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N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Ser.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in 50mM Tris, pH 8.0
- Endotoxin Level
- Less than 0.1 EU per μg of protein as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant rat IL-1α induces proliferation of D10.G4.1 (mouse helper T lymphocyte) in a dose-dependent manner. ED50 of this effect is 3-30 pg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Expressed in various immune cells, including macrophages and monocytes
- Function
- IL-1α is a potent proinflammatory cytokine that mediates inflammation, microbial invasion and tissue injury
- Interaction
- Interacts with many cell types, vascular endothelial cells and cancer associated fibroblasts in tumor microenvironment
- Ligand/Receptor
- Type I and type II interleukin-receptor
- Bioactivity
- Recombinant rat IL-1α induces proliferation of D10.G4.1 (mouse helper Tlymphocyte) in a dose-dependent manner. ED50 of this effect is 3-30 pg/mL.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Dinarello CA. 1996. Blood. 87:2095-2147.
- Carruth LM, et al. 1991. J Biol Chem. 266:12162-12167.
- Stevenson FT, et al. 1997. Proc Natl Acad Sci USA. 94:508-513.
- Bae JY, et al. 2014. Neoplasia. 16:928-938.
- Malik A. et al. 2018. Immunol. Res. 281:124–137.
- Melisi D, et al. 2009. Mol. Cancer Res. 7:624–633.
- Bretheau F, et al. 2022. Nat. Commun. 13:5786.
- Cohen I, et al. 2015. Sci. Rep. 5:14756.
- Gene ID
- 24493 View all products for this Gene ID
- UniProt
- View information about IL-1alpha on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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