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Purified anti-S100A8 Antibody

Pricing & Availability
Clone
A22124B (See other available formats)
Regulatory Status
RUO
Other Names
S100 Calcium-binding protein A8, Cystic Fibrosis Antigen (CFAG), Calgranulin A (CAGA, CGLA), Myeloid-related protein 8 (MRP8), S10A8
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. A22124B_PURE_S100A8_ICFC_1_063025
Human peripheral blood mononuclear cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with purified anti-human S100A8 (clone A22124B) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then stained with anti-human CD14 (clone HCD14) Pacific Blue™.
  • a. A22124B_PURE_S100A8_ICFC_1_063025
    Human peripheral blood mononuclear cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with purified anti-human S100A8 (clone A22124B) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then stained with anti-human CD14 (clone HCD14) Pacific Blue™.
  • b. A22124B_PURE_S100A8_ICFC_2_063025-2
    Human peripheral blood granulocytes were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with purified anti-human S100A8 (clone A22124B) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then stained with anti-human CD15 (clone W6D3) Pacific Blue™.
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377103 100 µg 332€
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Description

S100A8 is a member of the S100/calgranulin family of proteins containing 2EF-hand calcium-binding motifs. S100A8 is mainly expressed in cells of the myeloid lineage and is present in circulating neutrophils and monocytes. S100A8 expression is also transiently induced in keratinocytes after epidermal injury and in psoriasis where pro-inflammatory cytokines induce its expression. S100A8 is mostly cytoplasmic, but can be translocated to the plasma membrane or released into the extracellular environment upon cellular activation. S100A8 preferentially interacts with S100A9 to form a heterodimer S100A8/A9, also known as calprotectin. S100A8/A9 functions as a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. The extracellular functions of S100A8/A9 also include the recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. S100A8/A9 displays apoptosis-inducing activity against various tumor cells and also has antimicrobial activity by depriving bacterial pathogens of essential trace metals such as Zn2+ and Mn2+.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human S100A8
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3717176 (BioLegend Cat. No. 377103)

Antigen Details

Structure
Homodimer
Distribution

Neutrophils, monocytes, keratinocytes

Function
Pro-inflammatory molecule
Interaction
Neutrophils, monocytes
Ligand/Receptor
Toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (AGER)
Biology Area
Immunology
Antigen References
  1. Bhardwaj RS, et al. 1992. Eur. J. Immunol. 22:1891.
  2. Hunter MJ, et al. 1998. J. Biol. Chem. 273:12427.
  3. Hofmann MA, et al. 1999. Cell. 97:889-901.
  4. Cross SS, et al. 2005. Histopathology. 46:256.
  5. Zenz R, et al. 2005. Nature. 437:369.
  6. Hermani A, et al. 2006. Exp. Cell Res. 312:184.
  7. Vogl T, et al. 2007. Nature Med. 13:1042.
  8. Benedyk M, et al. 2007. J. Invest. Dermatol. 127:2001.
  9. Corbin BD, et al. 2008. Science. 319:962.
  10. Gebhardt C, et al. 2008. J. Exp. Med. 205:275.
  11. Loser K, et al. 2010. Nat Med. 16:713-17.
  12. Kang JH, et al. 2015. Immunology. 144:79-90.
Gene ID
6279 View all products for this Gene ID
UniProt
View information about S100A8 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/30/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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