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Alexa Fluor® 647 anti-JunB Antibody

Pricing & Availability
Clone
W20103F (See other available formats)
Regulatory Status
RUO
Other Names
Activator Protein 1, AP-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W20103F_A647_JunB_ICC_1_082925
HeLa cells (positive cell line, panel A) and NTERA-2 cells (low-expressing cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-JunB (clone W20103F). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 µm
  • A. W20103F_A647_JunB_ICC_1_082925
    HeLa cells (positive cell line, panel A) and NTERA-2 cells (low-expressing cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-JunB (clone W20103F). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 µm
  • B. W20103F_A647_JunB_ICC_2_082925
    HDLM-2 cells (high-expressing cell line, panel A) and NTERA-2 cells (low-expressing cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-JunB (clone W20103F). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 50 µm
  • C. W20103F_A647_JunB_ICFC_3_082925
    HeLa cells (positive cell line, filled histogram) and NTERA-2 cells (low-expressing cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-JunB (clone W20103F) or Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines).
  • d. W20103F_A647_JunB_ICFC_4_082925
    HDLM-2 cells (positive cell line, filled histogram) and NTERA-2 cells (low-expressing cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-JunB (clone W20103F) or Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines).
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600455 25 µg 120€
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600456 100 µg 296€
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Description

JunB is a member of the AP-1 family of transcription factors, which also includes c-Jun, c-Fos, Fra1, and JunD. JunB can form dimers with other Jun and Fos proteins, as well as members of the ATF transcription factor family, to form a functional AP-1 transcription factor complex. AP-1 complexes containing JunB can exert both activator and repressor function depending on the interacting partner and promoter sequence context. JunB displays both tumor suppressor and oncogenic activity, inducing genes involved in cell senescence and repressing genes involved in proliferation. JunB is required for T helper type 2 (Th2), T helper 17 (Th17) cell and regulatory T cell (Treg) differentiation. Multiple cancers display aberrant JunB expression and function. In some lymphomas, notably Hodgkin’s lymphoma, JunB expression is elevated and the protein functions as an oncogenic transcription factor. Conversely, loss of JunB is observed in chronic myeloid leukemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human JunB protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401).

RRID
AB_3720594 (BioLegend Cat. No. 600455)
AB_3720594 (BioLegend Cat. No. 600456)

Antigen Details

Structure
JunB is a 347 amino acid protein with a predicted molecular weight of 36 kD.
Distribution

Nucleus

Function
Transcription Factor
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Shaulian E and Karin M. 2002. Nat Cell Biol. 4:E131-6.
  2. Yang MY, et al. 2003. Blood. 101:3205-11.
  3. Carr T, et al. 2017. Nat Commun. 8:301.
  4. Katagiri T, et al. 2019. Mucosal Immunol. 12:1104-1117.
Gene ID
3726 View all products for this Gene ID
UniProt
View information about JunB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/29/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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