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Spark PLUS V450™ anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
W18272D (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG2b, κ
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Product Citations
publications
W18272D_SPKPLUSV450_IFN-y_FC_011426
C57BL/6 mouse splenocytes were treated with Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 4 hours and then fixed with Fixation Buffer (Cat. No. 420801) and permeabilized using Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse IFN-γ (clone W18272D) Spark PLUS V450™ (right) or stained with anti-mouse CD3ε (clone 145-2C11) FITC only (right).
  • W18272D_SPKPLUSV450_IFN-y_FC_011426
    C57BL/6 mouse splenocytes were treated with Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 4 hours and then fixed with Fixation Buffer (Cat. No. 420801) and permeabilized using Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse IFN-γ (clone W18272D) Spark PLUS V450™ (right) or stained with anti-mouse CD3ε (clone 145-2C11) FITC only (right).
Compare all formats See Spark PLUS V450™ spectral data
Cat # Size Price Quantity Check Availability Save
163515 25 µg 149 CHF
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163516 100 µg 329 CHF
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS V450™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS V450™ has a maximum excitation of 405 nm and a maximum emission of 452 nm.

Excitation Laser
Violet Laser (405 nm)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Distribution

Tregs

Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Ligand/Receptor
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Antigen References
1. Zaidi et al. 2011, Clin Cancer Res,17(19):6118-24
2. Castro et al. 2018, Front Immunol, 9:847
3. Alspach et al. 2019, Cold Spring Harb Perspect Biol, 11(3):a028480
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 1    Revision Date: 01.14.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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