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Purified anti-SOX9 Antibody

Pricing & Availability
Clone
W24113B (See other available formats)
Regulatory Status
RUO
Other Names
SOX-9, SRY-box transcription factor 9, Transcription factor SOX-9
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
a. W24113B_PURE_SOX9_Antibody_1_012126
Whole cell lysates from the indicated cell lines (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SOX9 antibody (clone W24113B). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Prime-Step™ Prestained Broad Range Protein Ladder (Cat. No. 773302)
  • a. W24113B_PURE_SOX9_Antibody_1_012126
    Whole cell lysates from the indicated cell lines (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SOX9 antibody (clone W24113B). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Prime-Step™ Prestained Broad Range Protein Ladder (Cat. No. 773302)
  • b. W24113B_PURE_SOX9_Antibody_2_012126
    HeLa (open histogram, negative control) and U-2 OS cells (filled histogram, positive control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-SOX9 (clone W24113B) or Purified Rat IgG2a, λ Isotype Control (open histogram, dashed line) (Cat. No. 402302) followed by Alexa Fluor® 647 Goat anti-rat IgG antibody (Cat. No. 405416).
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648707 25 µg 155 CHF
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648708 100 µg 425 CHF
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Description

SOX9 is a transcription factor of the SRY-related HMG box family that binds specific DNA motifs to control gene expression essential for chondrogenesis, testis development, neural differentiation, and maintenance of stem and progenitor cell lineages. It regulates expression by pathways such as Notch and Hedgehog and undergoes post-translational modifications, including phosphorylation and Sumoylation, that affect its nuclear localization, stability, and transcriptional potency. SOX9 is critically important for skeletal integrity and sexual differentiation; haploinsufficiency causes campomelic dysplasia and cleft palate. In adults, aberrant SOX9 expression contributes to organ fibrosis by promoting extracellular matrix production. Moreover, SOX9 dysregulation is implicated in diverse cancers, where it can enhance tumor progression, metastasis, and therapy resistance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SOX9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC- Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in Intracellular flow cytometry (ICFC), we recommend True-Phos™ Perm Buffer (Cat. No. 425401) for fixation and permeabilization.

Antigen Details

Structure
Human SOX9 protein is 509 amino acids length with a molecular weight of approximately 56.1 kDa
Distribution

Nucleus

Cell Type
Epithelial cells, Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Transcription Factors
Antigen References
  1. Ming Z, et al. 2022. Cell Mol Life Sci. 79:522.
  2. Panda M, et al. 2021. Biochim, Biophys Acta Rev Cancer. 1875:188517.
  3. Aldaz P, et al. 2020. Sci Rep. 10:357.
  4. Baumann K. 2024. Nat Rev Mol Cell Biol. 25:250.
Gene ID
6662 View all products for this Gene ID
UniProt
View information about SOX9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.21.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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