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Purified Anti-Musashi-2 Antibody

Pricing & Availability
Clone
W23272C (See other available formats)
Regulatory Status
RUO
Other Names
MSI2, MSI2H, Musashi-2, Musashi RNA Binding Protein 2, RNA-Binding Protein Musashi Homolog 2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W23272C_PURE_Musashi-2_WB_092625
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Musashi-2 (clone W23272C). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23272C_PURE_Musashi-2_WB_092625
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Musashi-2 (clone W23272C). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23272C_PURE_Musashi-2_IHC-P_092625
    IHC staining with Purified anti-Musashi-2 (clone W23272C) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) Purified anti-Musashi-2 (clone W23272C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 40X objective and merged. Scale bar: 100 µm
  • C. W23272C_PURE_Musashi-2_ICC_092625
    HAP1 WT cells (positive cell line, filled histogram) (Horizon Cat. No. C631) and HAP1 Musashi-2 Knockout cells (negative cell line, open histogram) (Horizon Cat. No. HZGHC006122c011) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified Anti-Musashi-2 (clone W23272C) or Purified Rat IgG2b, κ Isotype Control (dashed histrogram, representative of both the positive and negative cell lines) (Cat No. 400525), followed by Alexa Fluor® 647 Goat anti-Rat IgG (Cat. No. 405416).
  • D. W23272C_PURE_Musashi-2_ICFC_092625
    HAP1 MSI-2 KO cells (negative cell line, panel A) (Horizon Cat. No. HZGHC006122c011) and HAP1 WT cells (positive cell line, panel B) (Horizon Cat. No. C631) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100, and blocked with 5% FBS. Cells were then stained with Purified anti-Musashi-2 (clone W23272C), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
648878 25 µg 155 CHF
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648879 100 µg 435 CHF
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Description

Musashi-2 (MSI2) is a highly conserved RNA-binding protein that plays a critical role in post-transcriptional regulation of gene expression, particularly in stem cell maintenance, differentiation, and proliferation. Musashi-2 is essential for normal hematopoiesis and neural development, where it regulates the translation of mRNAs involved in cell cycle and developmental pathways. Dysregulation has been implicated in a variety of cancers, such as acute myeloid leukemia (AML) and MLL-rearranged acute lymphoblastic leukemia (ALL), where MSI2 supports leukemic stem cell survival and contributes to therapy resistance. Musashi-2 also promotes tumor progression in solid malignancies like pancreatic cancer, by modulating pathways such as PI3K/AKT/mTOR and epithelial-mesenchymal transition (EMT). Elevated Musashi-2 expression is frequently associated with poor prognosis, increased metastasis, and resistance to conventional therapies.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 MSI2 Knockout cell line (Horizon Cat. No. HZGHC006122c011)

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Musashi-2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3720622 (BioLegend Cat. No. 648878)
AB_3720622 (BioLegend Cat. No. 648879)

Antigen Details

Structure
Musashi-2 protein consists of 328 amino acids and has a molecular weight of approximately 35.2 kDa.
Distribution

Cytoplasm

Interaction
RHOBTB2, HNRNPH1 and HNRNPH2, MAPT (Tau protein), MEOX2, TIMM10B
Cell Type
Hematopoietic stem and progenitors, Leukemia, Monocytes, Neural Stem Cells, T cells
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References
  1. Huang L, et al. 2024. Cancer Cell Int. 24(1):273.
  2. Jiang L, et al. 2022. Front Oncol. 12:969632.
  3. Zang H, et al. 2025. Leukemia. 39(1):265.
  4. Taggart J, et al. 2016. Nat Commun. 7:10739.
Gene ID
124540 View all products for this Gene ID
UniProt
View information about Musashi-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.26.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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