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Purified anti-KLF4 Antibody

Pricing & Availability
Clone
W22026B (See other available formats)
Regulatory Status
RUO
Other Names
GKLF, EZF, Kruppel-like factor 4, Krueppel-like factor 4, Endothelial Kruppel-like zinc finger protein, KLF transcription Factor 4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22026B_PURE_KLF4_WB_073125
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-KLF4 (clone W22026B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22026B_PURE_KLF4_WB_073125
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-KLF4 (clone W22026B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22026B_PURE_KLF4_IHC-P_073125
    IHC staining with Purified anti-KLF4 on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panels B and C) Purified anti-KLF4 (clone W22026B) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (merged in panels A and B). Images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
648828 25 µg 175 CHF
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648829 100 µg 450 CHF
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Description

KLF4 (Krüppel-like factor 4) is a zinc finger transcription factor that plays a pivotal role in regulating diverse cellular processes, including proliferation, differentiation, apoptosis, and stem cell maintenance. It is one of the four Yamanaka factors (alongside Oct4, Sox2, and c-Myc) capable of reprogramming somatic cells into induced pluripotent stem cells. KLF4 can act as either a tumor suppressor or an oncogene depending on the tissue type and cellular environment. For instance, it suppresses tumor growth in colorectal and gastric cancers, while promoting tumorigenesis in breast and skin cancers. Its expression is tightly regulated at the transcriptional and post-translational levels, and it is influenced by signaling pathways such as Notch, Wnt/β-catenin, and TGF-β. Clinically, KLF4 has emerged as a potential prognostic biomarker and therapeutic target in various malignancies and its expression levels correlate with patient outcomes in cancers like renal clear cell carcinoma and glioblastoma, and it also modulates immune responses by enhancing CD8+ T cell differentiation and antitumor immunity. This dual role in tumor biology and immune regulation positions KLF4 as a key molecular bridge in cancer progression and therapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of Human KLF4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3720543 (BioLegend Cat. No. 648828)
AB_3720543 (BioLegend Cat. No. 648829)

Antigen Details

Structure
The human KLF4 protein is 513 amino acids length and has a predicted molecular weight of approximately 55 kDa.
Distribution

KLF4 is mainly located in the nucleus but may also be localized in the cytoplasm.

Interaction
• p300/CBP (CREB-binding protein): Acts as a transcriptional co-activator; KLF4 interacts with these to enhance gene expression
• Prmt1 (Protein arginine methyltransferase 1): Methylates KLF4, influencing its role in cell fate decisions and preventing primitive endoderm differentiation
• OCT4 (POU5F1) and SOX2: Core pluripotency factors; KLF4 interacts with them during somatic cell reprogramming
• MUC1: Interacts via KLF4’s C-terminal domain, potentially influencing epithelial cell signaling
• MEIS2 and PBX1: Homeobox transcription factors involved in development; KLF4 binds to specific isoforms of these proteins
Cell Type
Embryonic Stem Cells, Endothelial cells, Hematopoietic stem and progenitors, NK cells, NKT cells, T cells
Biology Area
Adaptive Immunity, Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cells, Transcription Factors
Antigen References
  1. Magnano DR, et al. 2025. Stem Cell Res. 86:103736.
  2. Zhao X, et al. 2025. Mol Biol Rep. 52(1):506.
  3. Yasuda NM, et al. 2025. FASEB J. 39(10):e70613.
  4. Ozturk S, et al. 2025. J Coll Physicians Surg Pak. 35(4):457.
Gene ID
9314 View all products for this Gene ID
UniProt
View information about KLF4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.31.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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