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Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
A20074H (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death protein 1, Protein PD-1, hPD-1, CD279
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20074H_PURE_CD279_DirecELISA_061725
Direct ELISA showing purified anti-human PD-1 (clone A20074H) bound to plate-immobilized recombinant human PD-1 (CD279)-Fc Chimera protein (Cat. Nos. 785102, 785104, 785106, and 785108). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A20074H_PURE_CD279_DirecELISA_061725
    Direct ELISA showing purified anti-human PD-1 (clone A20074H) bound to plate-immobilized recombinant human PD-1 (CD279)-Fc Chimera protein (Cat. Nos. 785102, 785104, 785106, and 785108). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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648811 100 µg 375 CHF
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PDL2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human PD-1 Recombinant Protein (His & Fc Tag)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20074H) could function as a capture antibody when paired with biotin anti-human PD-1 antibody (Cat. No. 621627) (clone A17188B) as the detection antibody and using Recombinant Human PD-1 (carrier-free) protein as the assay standard.

RRID
AB_3717175 (BioLegend Cat. No. 648811)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self
Interaction
APC, monocytes, dendritic cells, stromal cell, cancer cells
Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887.
  2. Fife BT, et al. 2009. Nature Immunol. 10:1185-92.
  3. Freeman GJ, et al. 2000. J Exp Med. 192:1027.
  4. Latchman Y, et al. 2001. Nat Immunol. 2001 2:261.
  5. Iwai Y, et al. 2002. Proc Natl Acad Sci USA. 99:12293-7.
  6. Dai S, et al. 2014. Cell Immunol. 290:72.
  7. Tan S, et al. 2017. Nature Commun. 8:14369.
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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