Loading...

ELISA MAX™ Deluxe Set Human Angiopoietin-1

Pricing & Availability
Regulatory Status
RUO
Other Names
ANG-1, KIAA0003, AGP1, AGPT, ANG1, ANGPT1
Ave. Rating
Submit a Review
Product Citations
publications
Human_Angiopoietin-1_ELISA-Kit_042425
  • Human_Angiopoietin-1_ELISA-Kit_042425
Cat # Size Price Quantity Check Availability Save
479004 5 plates 370 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Angiopoietin-1 encodes a 498 amino acid polypeptide with a molecular weight of 57 kDa. There are now four identified Angiopoietins: Angiopoietin-1, Angiopoietin-2, Angiotpoietin-3, and Angiopoietin-4. Angiopoietin-1 is a largely endothelial-specific growth factor/ligand/agonist that acts through the vascular tyrosine kinase receptor Tie-2. Angiopoietin-1 activates Tie-2 to initiate Angiopoietin/Tie-2 pathway. Signaling by Angiopoietin-1 promotes vascular endothelial cell survival and the sprouting and reorganizing blood vessels, as well as inhibiting activation of the vascular endothelial barrier to reduce leakage and leucocyte migration into tissues.

Structurally, Angiopoietin-1 has an N-terminal super clustering domain, a central coiled domain, a linker region, and a C-terminal fibrinogen-related domain responsible for the binding between the ligand and receptor (Tie-2). Angiopoietin-1 can form dimers, trimers, and tetramers. Angiopoietin-1 can form higher order multimers through its super clustering domain. However, not all the structures can interact with the tyrosine kinase receptor. The receptor can only be activated at the tetramer level.

BioLegend’s ELISA MAX™ Human Angiopoietin-1 Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human Angiopoietin-1. The set is designed for cost-effective and accurate quantification of human Angiopoietin-1 in serum, plasma, and cell culture supernatant. BioLegend’s ELISA MAX™ Human Angiopoietin-1 Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Human Angiopoietin-1 Capture Antibody (200x)
  • Human Angiopoietin-1 Detection Antibody (200x)
  • Human Angiopoietin-1 Lyophilized Standard
  • Avidin-HRP, 1000X
  • Assay Diluent B (5X) 
  • Substrate Solution F
  • Coating Buffer A (5X)
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4 
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1000 μL
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions 
  • Timer

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.70 ± 0.14 pg/mL
Standard Range
3.13 - 200 pg/mL

Antigen Details

Cell Type
Epithelial cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
NA
UniProt
View information about Angiopoietin-1 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 04.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account