Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Other Names
- Adipogenesis Inhibitory Factor (AGIF)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Cell-Vive™ GMP Recombinant Human IL-11 induces the proliferation of 7TD1 cells.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 585914 | 50 µg | 979 CHF | ||||
| 585915 | 250 µg | 2448 CHF | ||||
IL-11, a member of the IL-6 family of cytokines, exerts a wide range of biological effects on various cell types including hematopoietic cells, hepatocytes, adipocytes, neurons, and osteoblasts. IL-11 works synergistically with other growth factors including SCF, IL-4, IL-3, IL-7, IL-12, IL-13, and GM-CSF to stimulate the proliferation of cells from several hematopoietic lineages. Binding of IL-11 to the IL-11 receptor (IL-11R) induces membrane bound gp130 homodimerization and triggers STAT3 phosphorylation by JAK. IL-11 shares the common receptor subunit gp130 with IL-6, IL-27, LIF, OSM, CNTF, CT-1, CLC, and NP. Female mice deficient in IL-11R revealed an important role for IL-11 in embryonic implantation. In addition, IL-11 shows anti-inflammatory activity in models of inflammatory bowel disease, chemotherapy induced oral mucositis, and inflammatory arthritis. IL-11 and IL-13 are highly expressed in asthmatic airways (Th2 response), and IL-11 can inhibit Th1 responses and inhibits the production of Th1 cytokines such as IL-12 and shifts inflammation in Th2 direction. Elevated IL-11 expression is associated with tumor grade and invasion in gastric cancer. Recombinant human IL-11 has been clinically approved to improve platelet recovery after chemotherapy-induced thrombocytopenia.
Product DetailsBioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
- Low endotoxin level (≤ 0.1 EU/µg)
- Purity (≥ 95% or higher)
- Bioburden testing
- Mycoplasma testing
- Batch-to-batch consistency
- Vendor qualification
- Raw material traceability and documentation
- Documented procedures and employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Product Details
- Source
- Human IL-11, 180 amino acids Val20-Leu199 (Accession# P20809.1) was expressed in 293E.
- Molecular Mass
- The 180 amino acid recombinant protein has a predicted molecular mass of approximately 19.3 kD. The DTT-reduced and non reduced proteins migrate at approximately 19.3 kD by SDS-PAGE.
-
N-terminal
Sequence Analysis - Val-Ala-Pro-Gly-Pro-Pro-Pro-Gly-Pro-Pro
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- Protein was lyophilized from 0.1 μm solution containing PBS with 5% trehalose.
- Endotoxin Level
- Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
- Residual Host Cell Protein Content
- ≤ 0.500 ng/μg by ELISA
- Concentration
- 50 μg and 250 μg sizes are lyophilized
- Storage & Handling
- Unopened lyophilized vials can be stored at ≤ -20°C for 5 years and between 2°C – 8°C for 2 weeks. Reconstituted aliquots can be stored between -20°C to -70°C for 3 months and between 2°C – 8°C for 2 weeks. Reconstitute lyophilized protein in sterile PBS. Before reconstitution, make sure sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer to the vial, to obtain the recommended stock concentration of 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution.
- Activity
- Cell-Vive™ GMP Recombinant Human IL-11 induces proliferation of 7TD1 cells in a dose dependent manner. The ED50 for this effect is 0.83 – 8.3 ng/mL.
- Application
-
Bioassay
- Application Notes
-
Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech-BioLegend@revvity.com.
-
Application References
(PubMed link indicates BioLegend citation) -
- Singh A, et al. 2023. Invest Ophthalmol Vis Sci. 1:64. PubMed
- Disclaimer
-
BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Antigen Details
- Structure
- "Four-helix bundle" protein which folds similarly to IL-6
- Function
- IL-11, a multifunctional cytokine, plays important roles in hemopoiesis, thrombopoiesis, megakaryocytopoiesis, and bone resorption. It regulates macrophage differentiation and confers mucosal protection after chemotherapy and radiation therapy.
- Interaction
- Hematopoietic cells, hepatocytes, adipocytes, neurons, osteoblasts, fibroblasts, and gastrointestinal epithelial cells
- Ligand/Receptor
- Membrane bound or soluble IL-11Rα heterodimer with gp130β.
- Bioactivity
- Induces proliferation of 7TD1 cells in a dose dependent manner.
- Cell Sources
- IL-11 can be secreted by various cell types including epithelial, endothelial, keratinocytes, stromal, neuronal, fibroblasts, osteoclasts, and bone marrow stromal cells.
- Antigen References
-
- Putoczki T and Ernst M. 2010. J. Leukoc. Biol. 88:1109.
- Wilde MI and Faulds D. 1998. BioDrugs. 10:159.
- Barton VA. 2000. J. Biol. Chem. 275:36197.
- Robb L, et al. 1998. Nat. Med. 4:303.
- Lemoli RM, et al. 1995. Br. J. Haematol. 91:319.
- Elias JA, et al. 1994. J. Biol. Chem. 269:22261.
- Chen Q, et al. 2005. J. Immunol. 174:2305.
- Gene ID
- 3589 View all products for this Gene ID
- UniProt
- View information about IL-11 on UniProt.org
Follow Us