StarBright UltraViolet 795 anti-mouse/human CD44 antibody, CD44, IM7

Pricing & Availability

Clone: IM7 (See other available formats)
Regulatory Status: RUO
Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype: Rat IgG2b, κ
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Product Citations: publications

a.
IM7_SBUV795_CD44_FC_090225 — C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 17A2) APC and anti-mouse/human CD44 (clone IM7) StarBright UltraViolet 795 (left) or stained with anti-mouse CD3 (clone 17A2) APC only (right).

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103087

150 μg

255€

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.
Concentration: 0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186²⁰. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity¹, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Antigen Details

Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type: B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.
Gene ID: 12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt: View information about CD44 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD44 Reagents Request Custom Conjugation

Description	Clone	Applications
PE/Dazzle™ 594 anti-mouse/human CD44	IM7	FC
Brilliant Violet 711™ anti-mouse/human CD44	IM7	FC
APC/Fire™ 750 anti-mouse/human CD44	IM7	FC
TotalSeq™-A0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-C0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-B0073 anti-mouse/human CD44	IM7	PG
APC anti-mouse/human CD44	IM7	FC
Biotin anti-mouse/human CD44	IM7	FC,ICC,IHC-P
FITC anti-mouse/human CD44	IM7	FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44	IM7	FC
Purified anti-mouse/human CD44	IM7	FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44	IM7	FC
PE anti-mouse/human CD44	IM7	FC
Alexa Fluor® 488 anti-mouse/human CD44	IM7	FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44	IM7	FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44	IM7	FC
Alexa Fluor® 700 anti-mouse/human CD44	IM7	FC,SB
PE/Cyanine7 anti-mouse/human CD44	IM7	FC
APC/Cyanine7 anti-mouse/human CD44	IM7	FC
PerCP/Cyanine5.5 anti-mouse/human CD44	IM7	FC
PerCP anti-mouse/human CD44	IM7	FC
Brilliant Violet 421™ anti-mouse/human CD44	IM7	FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 785™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 510™ anti-mouse/human CD44	IM7	FC
Ultra-LEAF™ Purified anti-mouse/human CD44	IM7	FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44	IM7	FC
Alexa Fluor® 594 anti-mouse/human CD44	IM7	ICC,FC,IHC-P,SB
Purified anti-mouse/human CD44 (Maxpar® Ready)	IM7	FC,CyTOF®
Spark YG™ 570 anti-mouse/human CD44	IM7	FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44	IM7	FC
TotalSeq™-D0073 anti-mouse/human CD44	IM7	PG
Brilliant Violet 750™ anti-mouse/human CD44	IM7	FC
PerCP/Fire™ 806 anti-mouse/human CD44	IM7	FC
Spark Red™ 718 anti-mouse/human CD44	IM7	FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC
PE/Fire™ 810 anti-mouse/human CD44	IM7	FC
Spark UV™ 387 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC
Spark YG™ 581 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC
Spark NIR™ 685 anti-mouse/human CD44 (Flexi-Fluor™) Antibody	IM7	FC
StarBright UltraViolet 795 anti-mouse/human CD44	IM7	FC
PE/Fire™ 744 anti-mouse/human CD44	IM7	FC
Spark PLUS V450™ anti-mouse/human CD44	IM7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.23.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.