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- Regulatory Status
- RUO
- Other Names
- Monocyte chemotactic protein 1 (MCP-1), Immediate-early serum-responsive protein JE
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- Product Citations
- publications
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Recombinant rat CCL2 (MCP-1) chemoattract Baf3-mCCR2 transfectants in a dose-dependent manner. The ED50 for this effect is 0.15 – 1.5 ng/mL -
Stability Testing for Recombinant Rat CCL2. Recombinant rat CCL2 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to chemoattract Baf3-mCCR2 transfectants in a dose-dependent manner. The ED50 for this effect is 0.15 –1.5 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 720704 | 25 µg | 176€ | ||||
| 720706 | 100 µg | 440€ | ||||
CCL2, commonly known as MCP-1, is a member of the CC β chemokine family. It is widely expressed in endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β), and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of CCL2 in atherosclerosis. In addition, CCL2 has been implicated in monocytic infiltration of tissues during several inflammatory diseases and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, CCL2 plays a key role in the regulation of MMPs during transmigration. CCL2 induces migration of MonoMac6 by induction of MMP2. CCL2 and CCL3/MIP-1α mediate firm adherence and (subsequent) transmigration of neutrophils via protein synthesis and secondary generation of leukotrienes and PAF, which in turn directly activate neutrophils in mouse cremaster muscle. CCL2 is induced from endothelial cells by MIF and from fetal skin fibroblasts by IL-17.
Product DetailsProduct Details
- Source
- Rat CCL2 (MCP-1) amino acid (Gln24-Asn148) (Accession No P14844), was expressed in 293E cells.
- Molecular Mass
- The 125 amino acid recombinant protein has a predicted molecular mass of approximately 14.0 kD. The DTT-reduced and non-reduced proteins migrate at approximately 35 – 40 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant rat CCL2 (MCP-1) chemoattracts Baf3-mCCR2 transfectants in a dose-dependent manner. The ED50 for this effect is 0.15 – 1.5 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Secreted by fibroblasts, endothelial cells, smooth muscle cells, tumor cells, phytohemaglutinin stimulated mononuclear cells, TNF-α stimulated osteoblastic MC3T3-E1 cells
- Function
- MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1b, TNF, IFNg, PDGF, IL-4, MIF, and IL-17
- Interaction
- Monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells
- Ligand/Receptor
- CCR2
- Bioactivity
- Recombinant rat CCL2 chemoattracts Baf3-mCCR2 transfectants in a dose-dependent manner.
- Cell Type
- Endothelial cells, Lymphocytes, Macrophages, Microglia, Monocytes, Neurons, T cells
- Biology Area
- Immuno-Oncology, Immunology, Neurodegeneration, Neuroinflammation
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Jangani M, et al. 2022. J Leukoc Biol. 111:967. PubMed
- Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
- Wu W, et al. 2023. Mol Ther Nucleic Acids. 32:94. PubMed
- Wang Y, et al. 2021. Theranostics. 11:10074. PubMed
- Mitchell L, et al. 2013. Int Immunopharmacol. 15:357. PubMed
- Wang Y, et al. 2020. J Cereb Blood Flow Metab. 747:40. PubMed
- Tan X, et al. 2021. Mol Microbiol. 116:498. PubMed
- Kotla S, et al. 2017. J Biol Chem. 292:14885. PubMed
- Gene ID
- 24770 View all products for this Gene ID
- UniProt
- View information about CCL2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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