Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  4. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  5. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  6. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  7. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  8. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  9. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  10. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  11. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  12. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  13. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  14. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  15. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  16. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  17. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  18. Melendez E, et al. 2022. Development. 149: . PubMed
  19. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  20. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  21. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  22. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  23. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  24. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  25. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  26. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  27. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  28. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  29. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  30. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  31. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  32. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  33. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  34. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  35. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  36. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  37. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  38. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  39. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  40. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  41. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  42. Feng J, et al. 2022. Immunity. 55:405. PubMed
  43. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  44. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  45. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  46. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  47. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  48. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  49. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  50. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  51. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  52. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  53. Le DT, et al. 2023. iScience. 26:106059. PubMed
  54. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  55. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  56. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  57. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  58. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  59. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  60. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  61. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  62. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  63. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  64. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  65. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  66. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  67. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  68. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  69. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  70. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  71. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  72. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  73. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  74. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  75. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  76. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  77. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  78. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  79. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  80. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  81. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  82. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  83. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  84. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  85. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  86. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  87. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  88. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  89. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  90. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  92. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  93. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  94. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  95. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  96. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  97. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  98. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  99. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  100. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  101. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  102. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  103. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  104. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  105. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  106. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  107. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  108. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  109. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  110. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  111. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  112. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  113. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  114. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  115. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  116. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  117. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  118. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  119. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  120. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  121. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  122. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  123. Meng S, et al. 2022. Exp Anim. . PubMed
  124. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  125. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  126. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  127. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  128. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  129. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  130. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  131. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  132. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  133. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  134. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  135. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  136. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  137. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  138. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  139. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  140. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  141. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  142. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  143. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  144. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  145. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  146. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  147. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  148. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  149. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  150. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  151. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  152. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  153. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  154. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  155. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  156. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  157. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  158. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  159. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  160. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  161. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  162. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  163. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  164. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  165. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  166. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  167. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  168. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  169. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  170. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  171. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  172. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  173. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  174. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  175. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  176. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  177. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  178. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  179. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  180. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  181. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  182. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  183. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  184. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  185. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  186. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  187. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  188. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  189. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  190. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  191. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  192. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  193. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  194. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  195. Sci T 2013. Toxicol Sci. 136:72. PubMed
  196. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  197. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  198. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  199. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  200. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  201. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  202. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  203. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  204. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  205. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  206. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  207. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  208. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  209. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  210. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  211. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  212. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  213. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  214. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  215. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  216. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  217. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  218. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  219. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  220. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  221. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  222. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  223. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  224. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  225. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  226. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  227. Zhao J, et al. 2013. Elife. 21:537. PubMed
  228. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  229. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  230. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  231. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  232. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  233. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  234. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  235. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  236. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  237. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  238. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  239. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  240. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  241. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  242. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  243. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  244. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  245. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  246. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  247. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  248. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  249. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  250. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  251. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  252. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  253. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  254. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  255. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  256. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  257. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  258. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  259. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  260. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  261. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  262. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  263. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  264. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  265. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  266. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  267. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  268. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  269. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  270. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  271. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  272. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  273. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  274. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  275. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  276. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  277. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  278. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  279. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  280. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  281. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  282. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  283. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  284. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  285. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  286. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  287. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  288. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  289. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  290. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  291. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  292. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  293. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  294. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  295. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  296. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  297. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  298. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  299. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  300. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  301. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  302. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  303. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  304. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  305. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  306. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  307. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  308. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  309. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  310. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  311. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  312. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  313. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  314. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  315. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  316. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  317. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  318. Trial J, et al. 2020. Geroscience. . PubMed
  319. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  320. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  321. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  322. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  323. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  324. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  325. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  326. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  327. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  328. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  329. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  330. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  331. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  332. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  333. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  334. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  335. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  336. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  337. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  338. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  339. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  340. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  341. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  342. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  343. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  344. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  345. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  346. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  347. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  348. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  349. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  350. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  351. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  352. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  353. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  354. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  355. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  356. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  357. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  358. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  359. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  360. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  361. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  362. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  363. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  364. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  365. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  366. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  367. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  368. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  369. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  370. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  371. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  372. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed
  373. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  374. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  375. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  376. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  377. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  378. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  379. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  380. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  381. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  382. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  383. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  384. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  385. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  386. Wahane S, et al. 2021. Sci Adv. 7:. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA
Go To Top Version: 3    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account