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- Clone
- S23006A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
- Isotype
- Rat IgG1, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
BALB/c mouse splenocytes were stimulated with plate-bound anti-mouse TCR β (Cat. No. 109254) for 2 days, then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-mouse Granzyme B (clone S23006A) (left) or purified rat IgG1, λ isotype control (right), followed by anti-rat IgG1 PE. Cells were then stained with anti-mouse CD8a (clone 53-6.7) APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 168802 | 100 µg | 312€ | ||||
Granzyme B, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1, is a 32 kD serine protease. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clones S23006A and GB11 partially block each other.
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
-
Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
- Function
- Induction of cell death and apoptosis
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- Neutrophils, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
-
- Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
- Griffiths GM. and S. Isaaz. 1993. J. Cell Biol. 120:885.
- Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
- Wagner C, et al. 2008. Mol. Immunol. 45:1761.
- Gene ID
- 14939 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
Related FAQs
Other Formats
View All Granzyme B Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Granzyme B | S23006A | ICFC |
| PE anti-mouse Granzyme B | S23006A | ICFC |
| Alexa Fluor® 647 anti-mouse Granzyme B | S23006A | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Granzyme B
BALB/c mouse splenocytes were stimulated with plate-bound an... -
PE anti-mouse Granzyme B
BALB/c mouse splenocytes were stimulated with plate-bound an... -
Alexa Fluor® 647 anti-mouse Granzyme B
C57BL/6 mouse splenocytes were stimulated with plate-bound U...
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